Cat.-no	Description	Amount	Price €	Shop
20250	3D-Transfection Reagent Maximo	250 µl	109.00	add
20500	3D-Transfection Reagent Maximo	500 µl	199.00	add
21000	3D-Transfection Reagent Maximo	1 ml	369.00	add

3D Transfection reagent: manual
3D Transfection reagent: Deutsche Beschreibung

3D-Transfect Reagent is the newest reagent specifically developed to directly transfect cells cultured in 3D scaffold. 3D-Transfect Reagent is suitable for all kind of scaffolds and cells.

3D matrices not only add a third dimension to cells’ environment, they also allow creating significant differences in cellular characteristics and behavior. Because 3D matrices are routinely used in basic research and therapeutic applications, we have developed 3D-Transfect Reagent transfection reagent specific for scaffolds. In this way, scaffold-based 3D matrices combined with 3D-Transfect Reagent/DNA complexes, are colonized by cells to be transfected in a more natural environment. This method allows studying tissue engineering, tissue regeneration, tumor invasion, neural differentiation, cellular polarization, tissue formation, colonization, neurite growth…


3D-Transfect Reagentis the newest reagent specifically developed to directly transfect cells cultured in 3D scaffold.

This reagent is based on a novel technology that allows adding a third dimension to cell culture.

• Highly efficient: on cell lines and primary cells
• Specific for 3D scaffolds
• All type of nucleic acids: plasmid DNA, siRNA, shRNA...
• Completely biodegradable
• Serum compatible
• Long term protein expression

High transfection efficiency in various cells

3D-Transfection Reagentis highly efficient, even on primary cells and transgene expression is high and long.

Transfection of various cells on different scaffold




High secreted alkaline phosphatase expression




Efficient with various scaffolds and matrices


 

Name	Type of Scaffold
Collagen	Collagen-based scaffolds
Collagen-derived	Collagen-derived scaffolds
HA	Hyaluronic acid
Millicel (PTFE)	Cell culture inserts (Millipore)
PCL	Polycaprolactone
PEG	Poly (Ethylene Glycol)
PLGA	Poly (lactic-co-glycolic acid)
PS	Poly (styrene)
PU	Poly (urethane)

Outperforms competitors

Comparison of RFP production in HEK-293 cells




Comparison of secreted alkaline phosphatase production in COS-7 cells





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