Alu I
Sequence of AluI : 5’…AGCT…3’ 3’…TCGA…5’
| Catalog-no: |
Description |
units |
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| 250-101S |
Alu I |
600 units |
18.00
|
Alu I 600 units |
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|
250-101L
|
Alu I |
5x600 units |
90.00 |
Alu I 3000 units |
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AluI: Datasheet
AluI: Order by E-Mail
Overhang: blunt
Cut Site:
A G / C T
T C / G A
Isoschizomere: N/A
Source: Arthrobacter luteus.
Buffer supplied: 10x L
Substrate for unit definition: λ DNA.
Reaction conditions: 10 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37°C.
Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol.
Storage condition: Store at -20°C.
Absence of contaminants: Fifty units of Alu I do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA at 37°C. After ten-fold overdigestion with Alu I, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.
Heat inactivation: 65°C for 20 minutes.
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Table of Restriction Endonucleases
Table of Isoschizomeres
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