Cat.-no	Description	Amount	Price €	Shop
20101	Antibody delivery reagent 50-100 assays in 24-well plate	100 µl	209.00	add
20251	Antibody delivery reagent 125-250 assays in 24-well plate	250 µl	399.00	add
20501	Antibody delivery reagent 250-500 assays in 24-well plate	500 µl	699.00	add
21001	Antibody delivery reagent 500-1000 assays in 24-well plate	1 ml	1149.00	add

Antibody delivery reagent is the first dedicated intracellular antibody delivery reagent. This lipid-based formulation is the first serum compatible reagent allowing the delivery of functional antibodies into living cells.

Due to itsunique properties, Antibody delivery reagentforms non-covalent complexes with antibodiesthrough electrostatic and hydrophobic interactions. Consequently, chemical orgenetic couplings are not necessary. In addition, delivered antibodies retaintheir structure and function. This feature makes Antibody delivery reagentan exclusivereagent allowing the antibody to reach its intracellular target.

Antibody delivery reagentopens new fields of investigation in proteomics to elucidate complex molecularmechanisms or to design new potential therapies. For example, the intracellulardelivery of blocking antibodies could inhibit and/or neutralize proteinfunction. 

• Functionally active antibodies delivery
• Highly efficient in many primary cells and cell lines
• Serum compatible
• Biodegradable and no cytotoxicity
• Easy: straightforward protocol and ready-to-use

Intracellularlocalization of functional antibody

Antibody delivery reagentpermits the delivery of a functional antibody, making possible intracellularstaining and protein localization studies.

Anti-giantin and anti-NPC antibodies localization



Highlyefficient antibody transfection

Antibody delivery reagentpermits the delivery of antibodies in a large number of cells. It has been successfully tested on a large range of immortalized and primary cellsincluding primary neurons and glial cells.

Delivery of antibodies in HEK cells and L929 fibroblasts

 

Delivery reagents comparison

Incomparison with other protein delivery systems, Antibody delivery reagentdemonstrateshigher delivery efficiency inside living cells.

Anti-NPC and non specific FITC-IgG delivery comparison



Serumcompatible and fast delivery

Antibody delivery reagentis fully biodegradable and does not interfere with cellular mechanisms. Inaddition, it is compatible with serum containing culture media. You can obtaina significant amount of antibodies transported with no medium change which isless stressful for your cells. In vitroand in vivo experiments can bedirectly and quickly accomplished. Highest efficiencies can be achieved in lessthan 5 hours.

Delivery of proteins inside cells in presence of serum


 

 

Troubleshooting

Problems	Comments and Suggestions
Low delivery efficiency	1- Presence of BSA in your antibody solution. Make sure that the antibody is highly pure and devoid of additives such as BSA. 2-  AB-Delivery reagent amount. Optimize the quantity of AB-Delivery reagent as described in the table 3. 3- AB-Delivery reagent / antibody ratio. Optimize the  / antibody ratio within the range indicated in table 3. 4- Antibody amount. Use different quantity of antibody with the recommended or optimized AB-Delivery reagent / antibody ratio. 5- Cell density. A non-optimal cell density at the time of antibody delivery can lead to insufficient uptake. The optimal confluence should range from 50 to 70%. 6- Cell condition. 1) Cells that have been in culture for a long time (> 8 weeks) may become resistant to the delivery. Use freshly thawed cells that have been passaged at least once. 2) Cells should be healthy and assay during their exponential growing phase. The presence of contaminants (mycoplasma, fungi) alters considerably the delivery efficiency. 7- Cell culture medium composition. 1) For some cells, antibody delivery efficiency can be increased without serum or under reduced serum condition. Thus, assay these cells in serum-free medium during the first 4h of incubation. 8- Medium used for preparing AB-Delivery reagent / antibody complexes. It is critical that PBS is used during the preparation of the complexes. Do not use serum free medium, HBS or Tris buffer to prepare the complexes. 9- Incubation time and transfection volume. 1) The optimal time range between delivery and assay varies with cells, type of antibody, type of targeted proteins, etc. The delivery efficiency can be monitored after 4 to 96h. Fluorescently labeled antibody can be used to quantitatively monitored delivery kinetics. 2) To increase delivery efficiency, transfection volume suggested can be reduced for the first 4 to 24 hours. 10- Old  AB-Delivery reagent / antibody complexes. The  AB-Delivery reagent / antibody complexes must be freshly prepared every time. Complexes prepared and stored for more than 1 hour can be aggregated. 11- Positive control. Ensure that your experiment is properly set up and includes a positive control. The FITC-labeled IgG provided in the kit can be used as positive control for delivery efficiency. 12-  AB-Delivery reagent temperature. Reagents should have an ambient temperature and be vortexed prior to use. 13-  AB-Delivery reagent storage. Delivery efficiency can slowly decrease if  AB-Delivery reagent is kept more than one week at room temperature.
Cellular toxicity	1- Concentration of AB-Delivery reagent / antibody too high. Decrease the amount of  / antibody complexes added to the cells by lowering the antibody amount or the  reagent. Complexes aggregation can cause some toxicity; prepare them freshly and adjust the ratio as outlined previously. 2- Unhealthy cells. 1) Check cells for contamination, 2) Use new batch of cells, 3) Ensure culture medium condition (pH, type of medium used, contamination etc), 4) Cells are too confluent or cell density is too low, 5) Verify equipments and materials. 3- Antibody is cytotoxic. Use suitable controls such as cells alone,  AB-Delivery reagent alone or mock delivery (with positive IgG-FITC provided). 4- Incubation time. Reduce the incubation time of complexes with the cells. Delivery medium can be replaced by fresh medium after 3 to 24 h if necessary. 5- Antibody quality. Use high quality antibody as impurities could lead to cell death. 6- Key protein targeted. If the targeted protein is essential for cell survival this can lead to cell death. For instance as demonstrated with an anti-nuclear pore complex monoclonal antibody. In this way, the cell death is induced by the binding of antibody to the nuclear pore complexes.

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