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You are here : Ladders-Markers-DNA » Cloning Vectors / Phage DNA » Phage Lambda DNA

Phage Lambda DNA

Phage Lambda DNA is used in restriction enzymes research and for testing of restriction endonucleases activity.

Cat.-no Description Amount Price € Shop
 301-025  Phage Lambda DNA       500 µg     65,00  add
 301-100  Phage Lambda DNA  5x 500 µg   290.00  add

Phage Lambda DNA: Order by E-mail:
Phage Lambda DNA: Datasheet:
Phage Lambda DNA: Deutsche Beschreibung

Features:

λ DNA is used as one of the substrates in restriction enzymes research and for testing of restriction endonucleases activity.

Description/Preparation: 
The double stranded DNA is isolated from bacteriophage lambda (cl857 ind1 Sam7). The molecular weight is 31.5 x 106 daltons and it is 48,502 base pairs in length.
The phage is isolated from the heat inducible lysogen E. coli λ cl857 S7 by gel filtration. The DNA is isolated from the purified phage by phenol/chloroform extraction and dialyzed against 10 mM Tris-HCl (pH 7.4) and 1 mM EDTA.

Concentration:
    0.2-0.5 µg/µl

Storage Buffer:  10 mM Tris-HCl (pH 7.4), 1 mM EDTA.

Quality control: 
Gel analysis for purity. EcoRI and HindIII fragmentation patterns.
 
Transportation: Shipped on blue ice or room temperature

Storage: at -20°C for 24 months
Lambda DNA
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Overview

Overview: Vectors and Phages:

pBR322 cloning vector
pBR322 is a commonly used plasmid cloning vector in E. coli
pUC18 DNA
pUC18 is a commonly used plasmid cloning vector in E. coli.
Phage T7
Phage T7 DNA is an alternative to Lambda DNA for the restriction endonucleases
Phage Lambda DNA
Phage Lambda DNA is used in restriction enzymes research and for testing of restriction endonucleases activity.


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