qPCR/RTD PCR Mastermixes SaveLine: Eva for PCR cyclers with block techology	Short description
qPCR Master Mix E1 with EvaGreen	For quantitative Real-time PCR. EvaGreen intercalates during PCR. The Mastermix contains dUTP instead of dTTP. The Mix can be used with ROX dye as reference.
qPCR Master Mix E2 with UNG	For quantitative Real-time PCR. EvaGreen intercalates during PCR. The Mastermix contains dUTP instead of dTTP and UNG. The Mix can be used with ROX dye as reference.
qPCR Master Mix E3 with ROX	For quantitative Real-time PCR. EvaGreen intercalates during PCR. The Mastermix contains ROX as reference dye and dUTP instead of dTTP.
qPCR Master Mix E4 with ROX/UNG	For quantitative Real-time PCR. EvaGreen intercalates during PCR. The Mastermix contains ROX as reference dye, dUTP instead of dTTP and UNG.

qPCR Mastermixe SaveLine mit EvaGreen: Deutsche Beschreibung


Advantages of qPCR and RTD-PCR Mastermixes from GeneON:

- The Master Mixes contain dUTP. This prevent carry-over contamination of  previous PCR reactions using
  UDG treatment
- The Master Mixes contain a Hot-start enzyme which needs only a short first denaturation step of less
  than 5 minutes
- The Master Mixes are ready-to-use; all components: Hot-start enzyme, dATP, dCTP, dGTP, dUTP, 
   reaction buffer with KCl and MgCl₂, special enhancer and stabilizers are optimized.
- PCR-grade water is provided
- The amount of reactions is calculated for 50µl PCR assays


qPCR Mastermixe: Product overview

Real-time-PCR Master Mix E1

qPCR/RTD-PCR Master mix with EvaGreen and dUTP

Real-time PCR Master Mix E2

The master mix for RTD-PCR/qPCR contains: EvaGreen, dUTP and UNG

Real-time PCR Master Mix E3

The Master mix for RTD-PCR/qPCR contains: EvaGreen, dUTP and ROX (500 nM)

Real-time PCR Master Mix E4

The Master mix for RTD-PCR/qPCR contains: EvaGreen, dUTP. UNG and ROX (500 nM)

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