Cat.-no	Description	Amount	Price €	Shop
60250	VeroTrans 125 transfections with 1 µg of DNA	250 µl	129.00	add
60500	VeroTrans 250 transfections with 1 µg of DNA	500 µl	209.00	add
61000	VeroTrans 500 transfections with 1 µg of DNA	1 ml	399.00	add
65000	VeroTrans 2500 transfections with 1 µg of DNA	5 x 1 ml	1699.00	add

VeroTransfection reagent: Manual

Specific transfection reagent for Vero Cells

VeroTrans is a powerful reagent dedicated to the transfection of Vero cells.
VeroTrans is specifically designed to obtain highly efficient and reproducible transfection of Vero Cells. It can be used for many applications such as stable and transient transfection, protein and viral production…

• Optimized for Vero Cells
• Non toxic
• Serum compatible
• Simple, Ready-to-use and Rapid


High transfection efficiency in Vero cells

VeroTrans reagent has an exceptional capacity in destabilizing cell membranes, allowing the delivery of important DNA amounts into cells. Transfection efficiency exceeds most of others transfection reagents.
 
Transfection efficiency

Influence of cell number and DNA amount on transfection




Comparison with other transfection reagents

DNA transfection in Vero Cells




Percentage of transfected Vero Cells

 

Quality Controls

Each lot of VeroTrans reagent produced is qualified using rigorous standards. The following in vitro assays are conducted to qualify the function, quality and activity of each kit component. 

Specification	Standard Quality Controls
Purity	Silica Gel TLC assays. Every compound shall have a single spot.
Sterility	Thioglycolate assay. Absence of contamination shall be obtained for 7 days.
Biological Activity	Transfection efficacies on Vero cells. Every lot shall have an acceptance specification of > 85% of the activity of the reference lot

Optimization Protocol

Due to the variability of DNA, cells and culture conditions, it is complex to provide optimal guidelines. In this context, it might be required to accomplish few optimizations to achieve the best results.

1)    Quantity of DNA:In order to obtain the highest transfection efficiency, the amount of DNA used can be optimized (as detailed in table 3), especially with plasmids having a weak promoter or with large DNA vector such as BAC vectors or virus encoding vectors. These effects vary with the number of cells so, it is important to always keep the number of cells and the incubation time constant during your optimization procedure.

Table 3: DNA and VeroTrans reagent range for optimization.

 2)    Quantity of VeroTrans reagent :
After optimization of DNA amount, the ratio VeroTrans reagent / DNA can be optimized by varying the amount of VeroTrans reagent (see table 3) while maintaining the quantity of DNA constant. For instance, with 1µg of DNA, used 1, 2, 3 µL of VeroTrans reagent.

 3)    Cell number:
For stable transfection, cells can be seeded with lower density and, taking into account the efficiency of VeroTrans reagent, the quantity of DNA used can be reduced. 48 to 72 hours post-transfection, cells are transferred to fresh medium containing the appropriate antibiotics for selection. It is important to wait at least 48 hours before exposing the transduced cells to selection media.

4)    Incubation time:
The optimal time range between transfection and assay for gene activity varies with promoter activity, expression product, etc. The transfection efficiency can be monitored after 24 - 72 hours.

 

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