gDNA Removal Kit is designed for removal of contaminating gDNA from RNA prior to reverse transcription.
The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at moderate temperatures. This enables an inactivation step which is gentle enough to preserve both
quality and quantity of all present RNA.
Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.
The protocol is recommended for removal of genomic DNA from RNA preparations prior to reverse transcription.
Shipping: shipped on blue ice
Storage: store at -20°C, avoid frequent freeze / thaw cycles
Shelf Life: 16 months
Complete removal of genomic DNA from RNA preps
Most techniques used for RNA isolation yield RNA with signiﬁcant amounts of contaminating genomic DNA (gDNA), potentially resulting in false and inaccurate
mRNA quantiﬁcation. This is particularly a problem in the quantiﬁcation of low-copy transcripts or small samples. The gDNA Removal Kit efﬁciently removes gDNA from RNA preps to levels below the
detection limit of RT-qPCR (Figure).
gDNA Remover (red cap) - Heat-inactivatabledsDNase
Reaction Buffer (green cap) - 10xconcentration