Transportation: with blue ice
Storage: at 4°C for 3 months, at -20°C for more than 12 months
Manufactured and quality-controlled in accordance with ISO 9001 and 14001
Components | Volume per reaction | final conc. |
2X qPCR Master mix DLP2 with UDG |
25 µl | 1x |
Up-stream primer (10 µM stock) |
1,5 µl (range: 0,5-2.5 µl) | 300 nM |
Down-stream primer (10µM stock) |
1,5 µl (range: 0.5-2,5 µl | 300 nM |
Template DNA |
5 µl (0.1-15 ng/ml plasmid DNA) (1-10 µg/ml genomic DNA) |
< 500ng DNA |
Sterile dest. Water (included) |
up to 50 µl total reaction volume |
Step | Time | Temperature |
UNG treatment | 2 min | 50°C |
Initial denaturation | 2-3 min | 95°C |
35-50 Cycles: Denaturation Annealing Extension |
15-30 sec 30-65 sec 30 sec (per 500bp) |
95°C 55-65°C 72-75°C |
Note:
- an individual optimization of annealing temperature may be necessary for new combinations of primers
and Template DNA
Final price excl. shipping costs3
PCR MASTERMIX SELECTION TABLE
PCR Mastermixes with dTTP and SybrGreen
BioStar 2 PCR mastermix with SYBRGreen, blue dye, no
rox
BioStar 3 PCR mastermix with SYBRGreen, 60 nm LOW-ROX
BioStar 4 PCR mastermix with SYBRGreen, 900 nm HIGH-ROX
PCR Mastermixes with dTTP for probes
BioStar 5 PCR mastermix for probes
BioStar 6 PCR mastermix for probes 60 mM LOW-ROX
BioStar 7 PCR mastermix for probes 900 mM HIGH-ROX
PCR Master Mixes with Evagreen and dUTP
PCR Master Mix for qPCR EVA 1 dUTP+ EvaGreen
PCR Master Mix for qPCR EVA 2 +UNG
PCR Master Mix for qPCR EVA3 +ROX
PCR Master Mix for qPCR EVA4 +ROX+UNG
PCR Master Mix for qPCR EVA5 +low ROX
PCR Master Mix for qPCR E6 +low ROX+UNG
PCR Master Mixes for probes and dUTP
qPCR Master Mix DLP1 Exclusiv +dUTP
qPCR Master Mix DLP2 Exclusiv +dUTP+UDG
qPCR Master Mix DLP3/DLP5 Exclusiv ROX (500 nM, 100nM)
qPCR Master Mix DLP4/DLP6 Exclusiv +ROX 500nM or 100 nM+UDG