qPCR/real-time-PCR Master Mix DLP2

The master mix for qPCR contains: dUTP and UDG (for dual-labeled probes)

Features:
- optimized realtime PCR Mastermix using probe based detection (e.g.
FRET, Molecular Beacons or TaqMan)
- The Master mix contains dUTP instead of dTTP

- The Master contains UDG (Uracil-Glycosylase)
- The qPCR / RT-PCR Mastermix DLP1 is ready-to-use and is optimized
for high specificity and sensitivity because of optimized reaction buffer
- easy to use because ready-to-use Master Mix for block based PCR
Cycler
- The Master Mix can be used with ROX as reference dye (1x
concentrated)

Applications:
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination

Description:
The Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
The mix offer dUTP instead of dTTP to prevent carry-over contaminations of DNA from previous PCR reactions. It contains optimized UDG (Uracil-Glycosylase) for the treatment at the onset of thermal cycling.

Concentration: The Mastermix is 2x concentrated

List of components qPCR / RTD-PCR Master mix:
Hot-Start Polymerase for qPCR, dATP, dCTP, dGTP, dUTP, optimized reaction buffer with KCl and MgCl₂, Uracil-Glycosylase (UDG), stabilizers and enhancers, PCR-grade water

Transportation: with blue ice

Storage: at 4°C for 3 months, at -20°C for more than 12 months

Stability tests / Quality control / Comparison

Manufactured and quality-controlled in accordance with ISO 9001:2000

How to use the master mix for realtime PCR.

Components	Volume per reaction	final conc.
2X qPCR Master mix DLP2 with UDG	25 µl	1x
Up-stream primer (10 µM stock)	1,5 µl (range: 0,5-2.5 µl)	300 nM
Down-stream primer (10µM stock)	1,5 µl (range: 0.5-2,5 µl	300 nM
Template DNA	5 µl (0.1-15 ng/ml plasmid DNA) (1-10 µg/ml genomic DNA)	< 500ng DNA
Sterile dest. Water (included)	up to 50 µl total reaction volume

- vortex all solutions carefully before using and before PCR
- may you add the enzyme mix after Template DNA
- an individual optimization of annealing temperature may be
necessary for new combinations of primers and Template DNA

General Thermo-Cycler protocol:

Step	Time	Temperature
UNG treatment	2 min	50°C
Initial denaturation	2-3 min	95°C
35-50 Cycles: Denaturation Annealing Extension	15-30 sec 30-65 sec 30 sec (per 500bp)	95°C 55-65°C 72-75°C

Note:
- an individual optimization of annealing temperature may be necessary for new combinations of primers
and Template DNA

qPCR/real-time-PCR Master Mix DLP2

The master mix for qPCR contains: dUTP and UDG

* availibility of sample size may be limited

125,00 €

order / kaufen

Final price excl. shipping costs³

verfügbar / avaílable
1 - 3 Tage Lieferzeit / 1-3 days delivery¹

Related products

Ultra pure Nucleotides

PCR MASTERMIX SELECTION TABLE

PCR Mastermixes with dTTP and SybrGreen

BioStar 2 PCR mastermix with SYBRGreen, blue dye, no rox

BioStar 3 PCR mastermix with SYBRGreen, 60 nm LOW-ROX

BioStar 4 PCR mastermix with SYBRGreen, 900 nm HIGH-ROX

PCR Mastermixes with dTTP for probes

BioStar 5 PCR mastermix for probes

BioStar 6 PCR mastermix for probes 60 mM LOW-ROX

BioStar 7 PCR mastermix for probes 900 mM HIGH-ROX

PCR Master Mixes with Evagreen and dUTP

PCR Master Mix for qPCR EVA 1 dUTP+ EvaGreen

PCR Master Mix for qPCR EVA 2 +UNG

PCR Master Mix for qPCR EVA3 +ROX

PCR Master Mix for qPCR EVA4 +ROX+UNG

PCR Master Mix for qPCR EVA5 +low ROX

PCR Master Mix for qPCR E6 +low ROX+UNG

PCR Master Mixes for probes and dUTP

qPCR Master Mix DLP1 Exclusiv +dUTP

qPCR Master Mix DLP2 Exclusiv +dUTP+UDG

qPCR Master Mix DLP3/DLP5 Exclusiv ROX (500 nM, 100nM)

qPCR Master Mix DLP4/DLP6 Exclusiv +ROX 500nM or 100 nM+UDG

Deutsche Beschreibung

qPCR Mastermix DLP1

Datasheet qPCR-Mastermix DLP2

Material Safety Datasheet

References / Protocols / Notes / Recomendations / Tests

¹ Applies to shipping within Germany. Information about shipping policies for other countries can be found here: Payment and Delivery Information
² limitierter Sonderpreis / Special Price limited
³ Nettopreise ohne Umsatzsteuer zzgl. Frachtkosten / All prices without VAT excl. freight costs. :: MUSTER FÜR DEUTSCHLAND WERDEN KOSTENFREI GELIEFERT - AUCH WENN IM SHOP EIN PREIS AUSGEWIESEN WIRD :::

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qPCR/real-time-PCR Master Mix DLP2

The master mix for qPCR contains: dUTP and UDG (for dual-labeled probes)

Stability tests / Quality control / Comparison

How to use the master mix for realtime PCR.

The master mix for qPCR contains: dUTP and UDG