Features:
- optimized realtime PCR Mastermix using probe based detection (e.g.
  FRET, Molecular Beacons or TaqMan)
- The Master mix contains dUTP instead of dTTP

- The Master contains UDG (Uracil-Glycosylase)
- The qPCR / RT-PCR Mastermix DLP1 is ready-to-use and is optimized
   for high specificity and sensitivity because of optimized reaction buffer
- easy to use because ready-to-use Master Mix for block based PCR
  Cycler
- The Master Mix can be used with ROX as reference dye (1x
  concentrated)

Applications:
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination

Description:
The Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
The mix offer dUTP instead of dTTP to prevent carry-over contaminations of DNA from previous PCR reactions. It contains optimized UDG (Uracil-Glycosylase) for the treatment at the onset of thermal cycling.

Concentration: The Mastermix is 2x concentrated

List of components qPCR / RTD-PCR Master mix:
Hot-Start Polymerase for qPCR, dATP, dCTP, dGTP, dUTP, optimized reaction buffer with KCl and MgCl₂, Uracil-Glycosylase (UDG), stabilizers and enhancers, PCR-grade water

- vortex all solutions carefully before using and before PCR
- may you add the enzyme mix after Template DNA
- an individual optimization of annealing temperature may be
  necessary for new combinations of primers and Template DNA

General Thermo-Cycler protocol: