Manufactured and quality-controlled in accordance with ISO 9001 and 14001
Components | Volume per reaction | final conc. |
2X qPCR Master mix DLP4/DLP6 with UDG |
25 µl | 1x |
Up-stream primer (10 µM stock) |
1,5 µl (range: 0,5-2.5 µl) | 300 nM |
Down-stream primer (10µM stock) |
1,5 µl (range: 0.5-2,5 µl | 300 nM |
Template DNA |
5 µl (0.1-15 ng/ml plasmid DNA) (1-10 µg/ml genomic DNA) |
< 500ng DNA |
Sterile dest. Water (included) |
up to 50 µl total reaction volume |
Step | Time | Temperature |
UNG treatment | 2 min | 50°C |
Initial denaturation | 2-3 min | 95°C |
35-50 Cycles: Denaturation Annealing Extension |
15-30 sec 30-65 sec 30 sec (per 500bp) |
95°C 55-65°C 72-75°C |
Note:
- an individual optimization of annealing temperature may be necessary for new combinations of primers and Template DNA
* availibility of sample size may be limited
Final price excl. shipping costs3
PCR MASTERMIX SELECTION TABLE
PCR Mastermixes with dTTP and SybrGreen
BioStar 2 PCR mastermix with SYBRGreen, blue dye, no
rox
BioStar 3 PCR mastermix with SYBRGreen, 60 nm LOW-ROX
BioStar 4 PCR mastermix with SYBRGreen, 900 nm HIGH-ROX
PCR Mastermixes with dTTP for probes
BioStar 5 PCR mastermix for probes
BioStar 6 PCR mastermix for probes 60 mM LOW-ROX
BioStar 7 PCR mastermix for probes 900 mM HIGH-ROX
PCR Master Mixes with Evagreen and dUTP
PCR Master Mix for qPCR EVA 1 dUTP+ EvaGreen
PCR Master Mix for qPCR EVA 2 +UNG
PCR Master Mix for qPCR EVA3 +ROX
PCR Master Mix for qPCR EVA4 +ROX+UNG
PCR Master Mix for qPCR EVA5 +low ROX
PCR Master Mix for qPCR E6 +low ROX+UNG
PCR Master Mixes for probes and dUTP
qPCR Master Mix DLP1 Exclusiv +dUTP
qPCR Master Mix DLP2 Exclusiv +dUTP+UDG
qPCR Master Mix DLP3/DLP5 Exclusiv ROX (500 nM, 100nM)
qPCR Master Mix DLP4/DLP6 Exclusiv +ROX 500nM or 100 nM+UDG
qPCR Mastermix DLP4 / DLP6