qPCR/real-time-PCR Master Mix DLP4 / DLP6

Features:
- The PCR-Master mix contains dUTP instead of dTTP

- The PCR-Master contains UDG (Uracil-Glycosylase)
- The 2X PCR-Mix contains ROX (500nM, 100nM for LOW-ROX) as passive
   Reference dye (it provides a baseline in multiplex reactions)
- The qPCR / RTD-PCR Master mix DLP3/DLP5 is ready-to-use and is
  optimized for high specificity and sensitivity because of optimized
  reaction buffer
- easy to use because ready-to-use Master Mix

Applications:
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination

Description:
The Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
The mix offer dUTP instead of dTTP and UDG to prevent carry-over contaminations of DNA from previous PCR reactions. 

Concentration: The Mastermix is 2x concentrated

List of components qPCR / RTD-PCR Master mix:
Hot-Start Polymerase for qPCR, dATP, dCTP, dGTP, dUTP, UDG, ROX, optimized reaction buffer with KCl and MgCl₂, stabilizers and enhancers, PCR-grade water

Transportation: with blue ice

Storage: at 4°C for 3 months, at -20°C for more than 12 months,

Note: protect from Light

- vortex all solutions carefully before using and before PCR
- may you add the enzyme mix after Template DNA
- an individual optimization of annealing temperature may be
  necessary for new combinations of primers and Template DNA

General Thermo-Cycler protocol: