The "MB-Si" reagent kit I is used for the isolation of DNA or RNA from clinical material (oropharyngeal swab, nasopharyngeal swab, sputum) by a manual method.
Based on the reversible binding of nucleic acid molecules on the surface of magnetic sorbent particles in the presence of chaotropic salts, for subsequent detection and identification of NA by
reverse transcription polymerase chain reaction (RT-PCR), in particular for diagnostics of SARS-CoV-2 infection.
The sample is treated with a lysis solution in the presence of magnetic sorbent particles.
As a result, cell membranes, viral membranes and other biopolymer complexes are destroyed and nucleic acids are released.
Dissolved NA links with the sorbent particles, while the other components of the lysed biological material remain in solution and are removed during magnetic precipitation of sorbent on a
magnetic stand followed by washing of sorbent.
When an elution buffer is added to a magnetic sorbent, NA is eluted from the surface of the sorbent into solution, which is then separated from the sorbent particles by magnetic force.
As a result of this procedure, a highly purified NA sample is obtained, free from amplification reaction inhibitors, which ensures high analytical sensitivity of the RT-PCR study.
For professional use in clinical laboratory diagnostics and research practice. For in vitro diagnostics only.
Transport and storage:
For short time @ room temperature; 2-8°C for long term storage. Shelf-life: up to 14 months after delivery.