Cas9 Nuclease functional testing was done by in vitro DNA cleavage assay with the following protocol which gives more than 95% digestion of the substrate DNA as determined by
agarose gel electrophoresis.
1: 1 µl Cas9,
2: 2 µl Cas9
Cas9 nuclease is free from detectable RNase, Endonuclease (nicking) and non-specific DNase activities.