The enzyme mix is based on a new formulated reverse transcriptase, a Hot-Start Polymerase and RNase Inhibitor. The set is providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The reaction buffer includes extrapure dNTPs; ROX and reaction enhancers. The basis is a Real-time PCR with dual labeled probes and multiplexing capacity.
In the RT-step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. The hot-start polymerase activity is blocked.
In the first cycle of the PCR step the Hot-Start DNA polymerase activity is switched on and it synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template. The kit is optimized for all real-time PCR cyclers who are compatible with the evaluation of the ROX reference signal.
The One.step Realtime RT-PCR Master Mix from GeneON offers a tremendous convenience when applied to analysis of targets from multiple samples of RNA and minimizes the risk of contaminations.
Platforms: The Kit is suitable for all block-based Thermocycler. Stringent Quality Tests on ABI StepOne plus PCR Cycler
Components of Maximo.OneStep RT-qPCR Master Mix (for probes):
Enzyme-mix: HotStart Taq Polymerase, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer containing extra pure dNTPs and ROX
@ -20°C, avoid frequent thawing and freezing, store all components with ROX in the dark
the product will be shipped with "blue ice"
For optimal results it is recommended to make an individually optimization for each RNA / primer pair.
Manufactured and quality-controlled in accordance with ISO 9001:2000
Protocols / Manuals
The datasheet includes detailed protocols for:
1.) RT-PCR assay without sample denaturation (as a standard RNA/primer combinations)
2.) RT-PCR assay with sample denaturation (RNA/primer with a high degree of secondary
The enzyme mix is based on a genetically engineered reverse transcriptase with enhanced thermal stability; for highly structured and long cDNA fragments; includes ROX as Reference dye
* availibility of sample size may be limited
Final price excl. shipping costs3