LB Medium after the recipe of Lennox is a standard
medium for growth and propagation of E.
coli. The salt content is suitable for plasmid production, but enables as well
the addition of magnesium chloride by phage amplifications. Ideal for cultures, which need salt-sensitive antibiotica e.g. Zeocin.
Yeast extract 5 g/l
Recommended storage temperature of powder is +15 °C – +30
°C. Keep container tightly closed, because powder is very hygroscopic.
for 1 l of Medium:
1. Fill 900 ml water dest. in an 1-liter flask. Suspend 20 g powder medium while stirring.
2. Adjust to pH 7 with ca. 0.2 ml 5 N NaOH with stirring, fill up to a final volume of 1 liter.
Then autoclave medium for 20 min. at 121 °C.