Tris

(Molecular Biology Grade)

Synonym	2-Amino-2-Hydroxymethyl-1,3-Propanediol, THAM, Tris base, Tris-(Hydroxymethyl)-Aminomethane, Trometamol, Tromethamine
State of matter	Solid
Solubility (20°C)	800 g/L (H₂O)
useful pH range	pH 7.2 - 9.0
pK_a (20°C)	8.3
Melting point	168 - 173°C
Formula	C₄H₁₁NO₃
Mass	121.14 g/mol
CAS-No.:	77-86-1

EC-No.:	201-064-4
Storage:	RT
LGK:	10 - 13
Disposal:	28
Hazard pictogram(s)
Hazard statement(s)	H315-H319
Precautionary statement(s)	P302+P352-P305+P351+P338
Signal word	Warning
WGK:	1
Specification
Assay (titr.)	min. 99.9 %
pH (1 M; H₂O; 20°C)	10.5 - 11.5
Water	max. 0.2 %
Cu	max. 0.0001 %
Fe	max. 0.0001 %
Pb	max. 0.0001 %
A (1 cm/1 M in H₂O)
260 nm	max. 0.03
280 nm	max. 0.02
430 nm	max. 0.004

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Tris

Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0. It is used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, Tris can also be used for many custom running and loading buffers, most often with glycine and SDS. The use of single-junction pH electrodes that contain silver should be avoided when determining the pH of a solution containing Tris buffer.

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Tris

Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels.

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Tris

(Molecular Biology Grade)

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Deutsche Beschreibung

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Material Safety Datasheet

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