The molecule is double-stranded circle, 4361 bp in length. pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol. The molecular weight of pBR322 is 2.83x106 daltons. pBR322 is isolated from E. coli strain HB101 by a standard plasmid purification procedure.
Usage: pBR322 is a commonly used plasmid cloning vector in E. coli
Concentration: 0.5 µg/µl
Storage Buffer: 10 mM Tris-HCl (pH 8.0), and 1 mM EDTA.
Quality control: Gel analysis for purity. HinfI and AluI fragmentation patterns.
Transportation: Shipped on blue ice or room temperature
Storage: at -20°C for 24 months
pBR322 is a commonly used plasmid cloning vector in E. coli
* availibility of sample size may be limited
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