The molecule is double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC18 carries
a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. The molecular weight of pUC18 is 1.75x106
pUC18 is isolated from E. coli strain HB101 by a standard plasmid purification procedure.
Usage: pUC18 is a commonly used plasmid cloning vector in E. coli.
Concentration: 0.5 µg/µl
Storage Buffer: 10 mM Tris-HCl (pH 8.0), and 1 mM EDTA.
Quality control: Gel analysis for purity. BsiSI and AluI fragmentation patterns.
Transportation: Shipped on blue ice or room temperature
Storage: at -20°C for 24 months