EvaGreen Fluorescent DNA Stain is well known DNA intercalator dye specially developed for DNA analysis applications including real-time PCR (qPCR) and HRM-analysis. Upon binding to DNA, the non-fluorescent dye becomes highly fluorescent while showing no detectable inhibition to the PCR process.
The high quantum yield, excellent stability and lowest inhibition toward PCR makes it the ideal fluorophore in real-time PCR applications and a superior replacement for the widely used SYBR-Green I dye.
The dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling.
Shipping: blue ice or room-temperature
Storage Conditions: store at -20 °C and dark
Shelf Life: at least for 18 months
Supplied in 20 mM Tris-HCl pH 8.4, 0.1 mM EDTA and 0.01 % Tween-20
Concentration: 100 µM
Spectroscopic Properties: λexc 500 nm (bound to DNA); λem 530 nm (bound to DNA)
EvaGreen® DNA-binding dye is designed using a novel concept of DNA binding via “release-on-demand” mechanism (Fig. 1). The dye is constructed of two monomeric DNA-binding dyes linked by a flexible spacer. In the absence of DNA, the dimeric dye assumes a looped conformation that is inactive in DNA binding. When DNA is available, the looped conformation shifts via an equilibrium to a random conformation that is capable of binding to DNA to emit fluorescence. This chemical equilibrium provides a
unique mechanism to continuously supply the active form of the dye from the “reserve”(i.e., the dye in looped conformation), as more DNA is formed during a PCR process. Consequently, an EvaGreen® master mix can be formulated with relative high dye concentration to maximize fluorescence signal without PCR inhibition, making the mix suitable for both qPCR and HRM applications.*
Final price excl. shipping costs3
Eva Green Farbstoff