Reference dye for fluorescence signal normalization. The dye is optimized for block-based real-time PCR instruments that are compatible with the evaluation of
the FAM reference signal.
The dye is optimized for block-based real-time PCR instruments that are compatible with the evaluation of the FAM reference signal.
The fluorescent dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
The dye is supplied as 100x concentration.
Spectroscopic Properties: λabs 485 nm; λem 514 nm; 93.0 L mmol-1 cm-1 (pH 9.0); quantum yield 0.93 (pH 9.5)
Preparation of Mastermix for qPCR
Vortex the Fluorescein Reference Dye thoroughly prior to addition to the master mix. Add 0.5 µl Fluorescein Reference Dye for each 50 µl of master mix.
note: The preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors.
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C and darkness
Shelf Life: at least 18 months
Supplied in 20 mM Tris-HCl pH 8.4, 0.1 mM EDTA and 0.01 % Tween-20
Concentration: 100x conc
Stability tests / Quality Tests
Fluorescein Reference Dye 100x
Reference dye for fluorescence signal normalization