ROX reference dye is optimized for block-based real-time PCR instruments that are compatible with the evaluation of the ROX reference signal. The dye does not take part in the PCR reaction but
allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions.
Concentration: 50 x / 25 µM
Excitation maximum: Exλ = 576 nm
Emission maximum: Emλ = 601 nm
Preparation of the qPCR master mix:
The preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. Vortex the ROX reference dye thoroughly prior to addition to the master mix. Add 1 µl ROX
reference dye for each 50 µl of master mix.
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C and store dark
Shelf Life: at least for 16 months
Supplied in 20 mM Tris-HCl pH 8.4, 0.1 mM EDTA
and 0.01 % Tween-20