Used in single nucleotide polymorphisms and as 3´-end chain terminators in Sanger sequencing.
Stringent QC at ISO-certificated BULK-Production.
ddNTPs from GeneON in combination with modified Taq DNA Polymerase are used as 3'-end chain terminators in Sanger sequencing. @ pH 8,0 in water the high purity between
98 – 99 % (HPLC) offers a great performance in chain termination sequencing.
The nucleotides are especially manufactured and tested for application in sequencing reactions.
Application: 2‘,3‘-Dideoxynucleoside triphosphates inhibit the chain elongation of a given primer catalyzed by the DNA polymerase
(e.g. Klenow enzyme) and are therefore used for DNA sequencing according to Sange. Sequencing is achieved by including in each reaction a dideoxynucleotide that acts as a chain terminator. Four
reactions are set up, each containing the same template and primer but a chain terminator specific for A, C, G or T. Because only a small amount of the chain terminator is included, incorporation
into the new DNA strand is a random event. Each reaction therefore generates a collection of fragments, but every DNA strand will end at the same type of base (A, C, G or T).
Note: spin all reagents in the vial before opening and after setting up the Termination Mix
Transport: on blue ice or ambient temperature
Storage: few day at room temperature; for long term storage @ -20 °C
Stability tests / Quality Tests
Quality tests: The ddNTPs are tested: - for the absence the absence of DNases and RNases - for succesfully sequencing reaction - produced in German factory (ISO: 9001/2001)