Features:
- All components like: Taq polymerase, dNTP's, reaction buffers,
enhancers and stabilizers are lyophilized
- Transportation and at room-temperature
- simple, and fast setting up procedure for high yield and repeatable
results
Description:
Maximo Dry Master-Mix is optimized and ready-to-use mixture of all components for a successful PCR. Only your primers and your DNA Template
has to be added to the 20 µl assay.
Maximo Dry Master-Mix contains a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´
exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.
List of components:
12x8 lyophilized PCR-Tubes (0,2ml) "flat cap",
Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74°C
Quality control:
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 2 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination
Transportation: at room temperature
Storage: store at room-temperature in an aluminium coated bag or on another dry place; humidity < 65 % when sealing
is opended
Quality control:
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 2 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination
The lyophilized Mastemix is also available with HotStart Taq Polymerase.
Product-code: S295H
Components |
Volume per reaction |
1 Tube of Maximo Dry Mastermix |
|
Forward Primer: |
0,2-1 µM – 0,4-2µl / 10 µM |
Reverse Primer |
0,2-1 µM – 0,4-2µl / 10 µM |
Template DNA |
1-50 ng |
Sterile dest. Water (molecular grade) |
up to 20 µl total reaction volume |
Note:
- vortex all solutions and spin down carefully before using
Final price excl. shipping costs3
Dry Taq DNA. Pol DNA PreMix ready-to-use und gefriergetrocknet