- Anti-Taq (monoclonal antibody to Taq DNA polymerase) inhibits the
reaction of Taq DNA Polymerases at room temperature
- Ideal as enhancer for PCR reactions or for "homemade" Hot-Start
By preventing Taq polymerase activity until the denaturation step of PCR is underway, nonspecific amplification due to mispriming and/or formation of primer dimers during PCR assembly is prevented. As a result, experimental background is significantly decreased, and reaction specificity is increased.
Polymerase detergent/enhancer for:
- High specific amplification
- Multiplex amplification
- High sensitivity applications
- Low-copy number PCR
One unit is defined as the amount required to blocks 50% activity of 1 µg of Polymerase
By preventing Taq polymerase activity until the denaturation step of PCR is underway, nonspecific amplification due to mispriming and/or formation of primer dimers during PCR
assembly is prevented. As a result, experimental background is significantly decreased, and reaction specificity is increased.
Note: The ratio units/mg of polymerase varies (up to factor of 10). The binding rate Anti-Taq depends strongly of the polymerization region (active epitopes) of the polymerase. The optimized mixture has to be found in empiric test and for every new lot of polymerase, again.
Storage Buffer for S131/S132
20mM Tris-HCl (pH 7.0, at 22oC);50 mM KCl;0.1mM EDTA; 50% glycerol
Storage Buffer for S131/S132-8C1
3mM Tris-HCl (pH 8,0, @ 22oC); 15 mM KCl; 0.1mM EDTA; 20 % Trehalose (pH 8,0, @ 22oC);
Concentration: 2-10 mg/ml
2300 units of the specific polymerase activity are equal to 1 mg of antibodies
Storage: at – 20°C
Transportation: at room temperature or with blue ice
QC-Test: > 95% of protein in SDS electrophoresis in 15% PAAG
|Name||Final conc., M|
|Tris-HCl, pH 8,5||0,05-0,1|
|Glycerol, %||from 3 to 6|
|Tween 20, %||0,15-0,2|
|DFS-Taq polymerase, U/microliter||0,05-0,1|
|Trehalose MABs, mg/ml||0,0036-0,0072|
*TMAC - Tetramethylammonium chloride
The proportion DFS PLUS Taq / m-AntiTaq should be 1 Unit to 0,06-0,072 µg MAB
GeneON offers two types of Antibodies to Taq:
- Glycerol buffered
- Glycerol free buffered in Trehalose
Taq Antibody: Enhancer for Polymerase reaction at roomtemperature / monoclonal antibody to Taq DNA Polymerase
* availibility of sample size may be limited
NEW: Mixture of monoclonal Antibodies against Taq DNA Polymerase: ! CLEVER PRICE !
Optimized mixture of several monoclonal antibodies to Taq DNA polymerase. Stoichiometric complex that blocks the activity of Taq DNA Polymerases at room temperature. The Polymerase is released above the temperature of 60°C when the complete has been denaturated.
Inhibition of Taq DNA Polymerases activity at 55°C for 30 min
Storage and dilution buffer:
20 mM KCl, 10 mM Tris-HCl (pH 8.0)
Storage / Transportation:
@ -20 °C – at least 2 years
@ 2 to + 8°C – at least 6 months
@ RT about 2 weeks
Monoclonal Anti-Pfu DNA Polymerase (Antibodies to Pfu-DNA Polymerase)
Buffered in Trehalose.
Monoclonal anti-Pfu Polymerase antibodies (anti-Pfu Pol) were derived from hybridoma (fusion of mouse myeloma cell and cells after mouse immunization with Pfu DNA Polymerase (cloned). Anti-Pfu Pol antibodies (clone G11) are highly specific to Polymerization region of wild type Pfu, cloned Pfu; Pfu-based mutants , blocking polymerase activity at room temperature and up to 60 °C.
They binds to enzyme with high affinity, forming very temperature-stable protein/protein complex, which degradetes at the temperatures higher 70-72°C , liberating active Pfu polymerase into PCR reaction.
Addition Mabs to enzyme preparation allow avoiding miss-primimg and primer-dimer formation, increasing specificity of PCR and PCR product yield.
Ig subtype –IgG2b
1.) R.T. D'Aquila, L.J. Bechtel, J.A. Videler, Eron JJ, P. Gorczyca, J.C.Kaplan, Maximizing sensitivity and specificity of PCR by pre-amplification heating. Nucleic Acids Res. 19: 3749 (1991)
2.) Q. Chou, M. Russell, D.E. Birch, J. Raymond, W. Bloch. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res. 20: 1717-23 (1992)
3.) D.E. Kellogg , I. Rybalkin, S. Chen, N. Mukhamedova, T. Vlasik, P.D. Siebert, A. Chenchik. TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. Biotechniques. 16: 1134-7 (1994)