The Protein ladder EXtended contains 13 pre-stained proteins that resolve into sharp, tight bands in the range of 3,5-245 kDa. The Protein Ladder allows to monitor molecular weight separation
during electrophoresis, estimate molecular weights. The marker is ready-to-use. There is no need to boil. Easy to identify: Proteins are
covalently coupled with a blue chromophore except for two reference bands; green band at 25 kDa and red band at 75 kDa.
• Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.
• Monitoring of protein transfer onto membranes during Western blotting.
• Sizing of proteins on SDS-polyacrylamide gels and Western blots.
Usage of Protein ladder:
Mini gel application: 5 μl/well; 2.5 µl per well blots
Standard gel application: 10 μl/well; 5 µl per well for blots
Number of bands: 13 5, 11, 27, 20, 25, 35, 48, 63, 75, 100, 135, 180, 245 kDa