Source: An E.coli strain, that carries the cloned Aat II gene from Acetobacter aceti
Assayed on: Lambda DNA
Unit definition: One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-buffer: Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Activity in SEBuffers:
SEBuffer B 10 - 25%
SEBuffer G 25 - 50%
SEBuffer O 10 - 25%
SEBuffer W 25 - 50%
SEBuffer Y 100%
SEBuffer ROSE 50%
When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion.
Optimal temperature: 37°C
Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol.
Store at: -20°C.
Ligation: After 10-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents supplied with enzyme: 10 X SE-buffer Y
Methylation sensitivity: not tested
Inactivation: 20 minutes under 65°C
Notes: High enzyme concentration may result in star activity.
Final price excl. shipping costs3