Quality Control Assays
Ligation: After 2-fold overdigestion withAbs I, ~90% of DNA fragments can be ligated with T4 DNA Ligase and recut.
A 50 µl reaction containing 1 µg of pUC19SE/DriI and 2 units of enzyme incubated
for 16 hours resulted in the same pattern of DNA bands as a reaction incubated for 1
A long incubation time may result in star activity.
No detectable degradation of a single- and double-stranded oligonucleotide was observed
after incubation with 1 units of enzyme for 3 hours.