Source: An E.coli strain, that carries the cloned gene Mnl I from Moraxella nonliquefaciens
Assayed on: Lambda DNA
Unit definition: One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-buffer: G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA
Activity in SEBuffers:
SEBuffer B 75 - 100%
SEBuffer G 100%
SEBuffer O 25 - 50%
SEBuffer W 25 - 50%
SEBuffer Y 75 - 100%
SEBuffer ROSE 100%
When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion.
Optimal temperature: 37°C
Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol.
Store at: -20°C.
Ligation: After 10-fold overdigestion with enzyme 50% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents supplied with Enzyme: 10 X SE-buffer G, BSA
Methylation sensitivity: Blocked by overlapping CG methylation: CCTmCG.
Inactivation: 20 minutes under 65°C
Notes: To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
*availibility of sample size may be limited
Final price excl. shipping costs3