Psi I - TTA↑TAA - AAT↓ATT

Source: Pseudomonas species-SE-G49

 

 

 

 

 

 

 

Recognition site:

TTA↑TAA

AAT↓ATT

 

Source: Pseudomonas species-SE-G49

 

Assayed on: Lambda DNA

 

Unit definition: One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

 

Optimal SE-buffer: B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)

 

Activity in SEBuffers:

SEBuffer B              100%

SEBuffer G       25 -  50%

SEBuffer O       10 -  25 %

SEBuffer W       25 -  50%

SEBuffer Y        75 - 100%

SEBuffer ROSE         40%

 

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

 

Optimal temperature: 37°C

 

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol;

 

Store at: -20°C.

 

Ligation: After 5-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated and of these 95% can be recut.

 

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.

 

Reagents supplied with enzyme: 10 X SE-buffer B

 

Methylation sensitivity: not tested

 

Inactivation: 20 minutes under 65°C

 

Notes: The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,25.

 

E280 - Psi I - 1000 units

Psi I - TTA↑TAA - AAT↓ATT - Restriction Endonuclease from Sibenzyme

 

* availibility of sample size may be limited

168,00 €
order / kaufen
  • 5 - 8 Tage Lieferzeit1

Related products

 

 

 

 

 

Ultra pure Nucleotides


Datasheet

Material Safety Datasheet


References / Protocols / Notes / Recomendations / Tests

 

 

Matrix themes

Close