Source: An E.coli strain that carries the cloned PspX I gene from Pseudomonas species A1-1
Assayed on: Lambda DNA (HindIII-digest)
Unit definition: One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total
reaction volume of 50 μl.
Optimal SE-buffer: Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA