Source: An E.coli strain, that carries the cloned gene Xma I from Xanthomonas malvacearum.
Assayed on: Adenovirus -2 DNA
Unit definition: One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus -2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-buffer: Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Activity in SEBuffers:
SEBuffer B 75 - 100%
SEBuffer G 50 - 75%
SEBuffer O 0 - 10%
SEBuffer W 0 - 10%
SEBuffer Y 100%
SEBuffer ROSE 50%
When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion.
Optimal temperature: 37°C
Storage conditions: 20 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol.
Store at: -20°C.
Ligation: After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated. Of these 90% can be recut.
Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 37°C
Reagents supplied with enzyme: 10 X SE-buffer Y
Methylation sensitivity: not tested
Inactivation: 20 minutes under 65°C
Xma I - C↑CCGGG - GGGCC↓C - Restriction Endonuclease from Sibenzyme
* availibility of sample size may be limited
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