The presence of genomic DNA in RNA preparations is often a significant problem for downstream applications. This
contamination often leads to false-positive signals and misrepresentation of gene expression levels.
gDNA Removal Kit from GeneON removes gDNA from RNA prior to reverse transcription.
The Removal Kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at moderate
After that inactivation step which is gentle enough to preserve both quality and quantity of all present RNA.
Reverse Transcription can be performed in the same
tube, thereby minimizing pipetting steps and reducing hands-on time.
Shipping: shipped on blue
Storage: store at -20°C, avoid
frequent freeze / thaw cycles
Shelf Life: 16 months
Complete removal of genomic DNA from RNA preps
Most techniques used for RNA isolation yield RNA with signiﬁcant amounts
of contaminating genomic DNA (gDNA), potentially resulting in false and inaccurate mRNA quantiﬁcation. This is particularly a problem in the quantiﬁcation of low-copy transcripts or small
samples. The gDNA Removal Kit efﬁciently removes gDNA from RNA preps to levels below the detection limit of RT-qPCR (Figure).
gDNA Remover reagent
(red cap) - Heat-inactivatable dsDNase
10x Reaction Buffer