The presence of genomic DNA in RNA preparations is often a significant problem for downstream applications. This contamination often leads to false-positive signals and misrepresentation of gene expression levels.
gDNA Removal Kit from GeneON removes gDNA from RNA prior to reverse transcription.
The Removal Kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at moderate temperatures.
After that inactivation step which is gentle enough to preserve both quality and quantity of all present RNA.
Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time.
Shipping: shipped on blue ice
Storage: store at -20°C, avoid frequent freeze / thaw cycles
Shelf Life: 16 months
Complete removal of genomic DNA from RNA preps
Most techniques used for RNA isolation yield RNA with signiﬁcant amounts of contaminating genomic DNA (gDNA), potentially resulting in false and inaccurate mRNA quantiﬁcation. This is particularly a problem in the quantiﬁcation of low-copy transcripts or small samples. The gDNA Removal Kit efﬁciently removes gDNA from RNA preps to levels below the detection limit of RT-qPCR (Figure).
gDNA Remover reagent
(red cap) - Heat-inactivatable dsDNase
10x Reaction Buffer
Perform the assay set-up as following:
|10x Reaction Buffer||1 µl||2 µl||5 µl|
|gDNA Remover||1 µl||1 µl||1 µl|
|your RNA preparation||8 µl||17 µl||44 µl|
- 10 min @ 37°C or 20 min at 25°C
- 5 min @ 58°C for inactivation of the enzyme
The reverse transcription reagents can be directly added to the tube containing the puriﬁed RNA sample, after completion of DNase treatment,
Final price excl. shipping costs3
Overview PCR Master Mixe:
BioStar 2 PCR mastermix with SYBRGreen blue dye, no rox
BioStar 3 PCR mastermix with SYBRGreen 60 nm LOW-ROX
BioStar 4 PCR mastermix with SYBRGreen 900 nm HIGH-ROX
BioStar 5 PCR mastermix
BioStar 6 PCR mastermix
for probes 60 mM LOW-ROX
BioStar 7 PCR mastermix
for probes 900 mM HIGH-ROX
gDNA "removal" Kit