One.Step RT-PCR Master Mix with EvaGreen

The superior PCR Master mix is based on a genetically engineered reverse transcriptase; for improved efficiency; structured and long cDNA fragments; contains EvaGreen as intercalating dye

Features:

The kit contains all reagents required for RT-qPCR in a set to ensure fast and easy preparation with a minimum of pipetting steps. Just add template, primers. That's all.
The One.Step RT-PCR Kit is highly sensitive: less than 10 pg to 100 ng total RNA or < 1 pg to 20 ng poly(A) RNA (mRNA) can be detected when using highly expressed transcripts

Description:
The enzyme mix is based on a new formulated reverse transcriptase, a Hot-Start Polymerase and RNase Inhibitor. The set is providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The reaction buffer includes extrapure dNTPs, EvaGreen as intercalating dye and reaction enhancers.

Principal:
In the RT-step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. The hot-start polymerase activity is blocked.
In the first cycle of the PCR step the Hot-Start DNA polymerase activity is switched on and it synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template.

The One.step Realtime RT-PCR Master Mix from GeneON offers a tremendous convenience when applied to analysis of targets from multiple samples of RNA and minimizes the risk of contaminations.

Platforms: The Kit is suitable for all block-based Thermocycler. Stringent Quality Tests on ABI StepOne plus PCR Cycler

Components of Maximo.OneStep RT-qPCR:
Enzyme-mix: HotStart Taq Polymerase, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer, extra pure dNTPs, EvaGreen DNA and stabilizers

RNase-free water

Storage:
@ -20°C, avoid frequent thawing and freezing, store all components with EvaGreen in the dark

Transport:
the product will be shipped with "blue ice"

Stability tests / Quality control / Comparison

Manufactured and quality-controlled in accordance with ISO 9001:2000

The PCR mastermix for reverse transcriptase provided increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments.

Protocols / Manuals
The datasheet includes detailed protocols for:

1.) RT-PCR assay without sample denaturation (as a standard RNA/primer combinations)

2.) RT-PCR assay with sample denaturation (RNA/primer with a high degree of secondary
structure)

2.a) Preparation of RNA / Primer Mix
2.b) Denaturation and primer annealing
2.c) Preparation of the RT-PCR Mix

3.) Reverse Transcription and thermal cycling

One.Step RT-PCR Master Mix with EvaGreen

Great Mastermix for RT-realtime PCR based on a genetically engineered reverse transcriptase; for improved efficiency, thermostability and specivicity; structured and long cDNA fragments; contains EvaGreen as intercalating dye and ROX as reference

* availibility of sample size may be limited

375,00 €

order / kaufen

Final price, free shipping to selected countries³

Free shipping to the following countries: Germany Show more Show less

verfügbar / avaílable
1 - 3 Tage Lieferzeit / 1-3 days delivery¹

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Ultra pure Nucleotides

Deutsche Beschreibung

One.Step RT-PCR Master Mix mit EvaGreen

Datasheet One.Step RT-PCR Master Mix with EvaGreen

Material Safety Datasheet

References / Protocols / Notes / Recomendations / Tests

¹ Applies to shipping within Germany. Information about shipping policies for other countries can be found here: Payment and Delivery Information
² limitierter Sonderpreis / Special Price limited
³ Nettopreise ohne Umsatzsteuer zzgl. Frachtkosten / All prices without VAT excl. freight costs. :: MUSTER FÜR DEUTSCHLAND WERDEN KOSTENFREI GELIEFERT - AUCH WENN HIER EIN PREIS AUSGEWIESEN WIRD :::

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