One.Step RT-PCR Master Mix with EvaGreen
The superior Mastermix is based on a genetically engineered reverse transcriptase; for improved efficiency; structured and long cDNA fragments; contains EvaGreen as intercalating dye
Features:
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The kit contains all reagents required for RT-qPCR in a set to ensure fast and easy preparation with a minimum of pipetting steps. Just
add template, primers. That's all.
- The One.Step RT-PCR Kit is highly sensitive: less than 10 pg to 100 ng total RNA or < 1 pg to 20 ng poly(A) RNA (mRNA) can be detected when using highly expressed transcripts
The enzyme mix is based on a new formulated reverse transcriptase, a Hot-Start Polymerase and RNase Inhibitor. The set is providing increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The reaction buffer includes extrapure dNTPs, EvaGreen as intercalating dye and reaction enhancers.
Principal:
In the RT-step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. The hot-start polymerase activity is blocked.
In the first cycle of the PCR step the Hot-Start DNA polymerase activity is switched on and it synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template.
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The One.step Realtime RT-PCR Master Mix from GeneON offers a tremendous convenience when applied to analysis of targets from multiple samples of RNA and minimizes the risk of contaminations. |
Platforms: The Kit is suitable for all block-based Thermocycler. Stringent Quality Tests on ABI StepOne plus PCR Cycler
Components of Maximo.OneStep RT-qPCR:
Enzyme-mix: HotStart Taq Polymerase, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer, extra pure dNTPs, EvaGreen DNA and stabilizers
RNase-free water
Storage:
@ -20°C, avoid frequent thawing and freezing, store all components with EvaGreen in the dark
Transport:
the product will be shipped with "blue ice"
Stability tests / Quality control / Comparison
Manufactured and quality-controlled in accordance with ISO 9001:2000
Protocols / Manuals
The datasheet includes detailed protocols for:
1.) RT-PCR assay without sample denaturation (as a standard RNA/primer combinations)
2.) RT-PCR assay with sample denaturation (RNA/primer with a high degree of secondary
structure)
2.b) Denaturation and primer annealing
2.c) Preparation of the RT-PCR Mix
3.) Reverse Transcription and thermal cycling
Great Mastermix for RT-realtime PCR based on a genetically engineered reverse transcriptase; for improved efficiency, thermostability and specivicity; structured and long cDNA fragments; contains EvaGreen as intercalating dye and ROX as reference
* availibility of sample size may be limited
- verfügbar / avaílable
Deutsche Beschreibung
One.Step RT-PCR Master Mix mit EvaGreen
Datasheet One.Step RT-PCR Master Mix with EvaGreen
Material Safety Datasheet
References / Protocols / Notes / Recomendations / Tests
Contact
GeneOn GmbH
Paracelsusstrasse 10
67071 Ludwigshafen am Rhein
Deutschland / Germany
Phone: +49-621-5720 864
Fax: +49-621-5724 462
info@geneon.net
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See Categories
- DNA/RNA Extraction
- Real-time PCR
- RT-qPCR - Master Mixes
- PCR / DNA Amplification
- RT-PCR - Reverse Transcription
- Nucleotides, dNTPs, Primers
- Marker / Gel Staining.
- Enzymes and Finechemicals
- Fluorescent Dyes / supplements
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- PCR-96 plates and tools, Tips, Tubes, Filtration
- Restriction Endonucleases
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GENEON GmbH - Germany - Paracelsusstrasse 10 - 67071 Ludwigshafen - www.geneon.net - info@geneon.net - Tel: 0621-5720864