Features:

• The kit contains all reagents required for RT-qPCR in a set to ensure fast and easy preparation with a minimum of pipetting steps. Just add template, primers. That's all.
   
• The One.Step RT-PCR Kit is highly sensitive: less than 10 pg to 100 ng total RNA or < 1 pg to 20 ng poly(A) RNA (mRNA) can be detected when using highly expressed transcripts

Platforms: The Kit is suitable for all block-based Thermocycler. Stringent Quality Tests on ABI StepOne plus PCR Cycler

Components  of Maximo.OneStep RT-qPCR:
Enzyme-mix: HotStart Taq Polymerase, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer, extra pure dNTPs, EvaGreen DNA and stabilizers

RNase-free water

Storage:
@ -20°C, avoid frequent thawing and freezing, store all components with EvaGreen in the dark

Transport:
the product will be shipped with "blue ice"

Manufactured and quality-controlled in accordance with ISO 9001:2000

Protocols / Manuals
The datasheet includes detailed protocols for:

1.) RT-PCR assay without sample denaturation (as a standard RNA/primer combinations)

2.) RT-PCR assay with sample denaturation (RNA/primer with a high degree of secondary
structure)