The One.step Realtime RT-PCR Master Mix from GeneON offers a tremendous convenience when applied to analysis of targets from multiple samples of RNA and minimizes the risk of contaminations.
Platforms: The Kit is suitable for all block-based Thermocycler. Stringent Quality Tests on ABI StepOne plus PCR Cycler.
Storage: @ -20°C, avoid frequent thawing and freezing, store all components with EvaGreen / ROX in the dark
Transport: the product will be shipped with "blue ice"
For optimal results it is recommended to make an individually optimization for each RNA / primer pair.
Manufactured and quality-controlled in accordance with ISO 9001:2000
Components of Maximo.OneStep RT-qPCR Mix:
Enzyme-mix: HotStart Taq Polymerase, Reverse Transcriptase, RNase Inhibitor and enhancers, Reaction buffer, extra pure dNTPs, EvaGreen DNA dye and ROX
Protocols / Manuals
The datasheet includes detailed protocols for:
1.) RT-PCR assay without sample denaturation (as a standard RNA/primer combinations)
2.) RT-PCR assay with sample denaturation (RNA/primer with a high degree of secondary
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