- blue/white screening
- distinguish between positive and negative clones after transformation
X-Gal is a highly pure molecular biology grade chemical for colorimetric detection of b-galactosidase activity. It is used for “blue/white screening” of
bacterial colonies to differ bacterial or phage clones after transformation. In combination with suitable bacterial cloning vectors, host strains, and IPTG, X-Gal provides an easy way to
distinguish between positive and negative clones after transformation.
Purity of >98% by HPLC. Tested in the blue/white colony screening experiment.
- Preparation of a 20 mg/ml stock solution in dimethylformamide or dimethylsulfoxide is recommended.
- Dimethylformamide dissolves some plastic materials. The direct addition of dimethylformamide containing solution to plastic Petri dishes should be avoided.
at - 20°C in the dark; shipped at ambient temperature