Agarose for best results in gel-electrophoresis

Agarose for electrophoresis in gels has an excellent price. Standard Agarose ist ideal for use for analytical as well as preparative nucleic acid electrophoresis of DNA fragments from 50 bp to 50 kbp.

Agarose for Gelelectroporesis — Agarose - best results for gelelectroporese

Features Agarose:
- High separation properties and sharp band patterns
- Easy solubility without foaming
- Excellent optical transparency

Applications:
- Separation of PCR products
- DNA: approx. 0.05 kbp – 50 kbp
- RNA: approx. 0.30 kb – 20 kb

Specifications Agarose:
- Gelling temperature: ≤ 37 °C
- Melting temperature: ≤ 90 °C
- Electroendosmosis: ≤ 0.140
- Gel strength (1.5 %): ≥ 2300 g / cm2
- Sulphate content: ≤ 0.10 %
- Water content: ≤ 10.0 %

Agarose, Electrophoresis, Gel of Agarose — Standard Agarose for Gelelekrophoresis

Agarose Stability tests / Quality Tests

Quality Assurance:
'Molecular Biology Grade'
- Certified free of DNases and RNases
- No DNA binding
- High lot-to-lot consistency

Quality control of Agarose:

'Molecular Biology Grade'
- Certified free of DNases and RNases
- No DNA binding
- High lot-to-lot consistency

Even at low concentrations the agarose gel produced is very firm

Agarose gel bath — How to run electrophoresis with agarose gel

Agarose for gel-electrophoresis

Universal Agarose for gel-electrophoresis and an excellent price is ideal for analytical as well as preparative nucleic acid electrophoresis of fragments from 50 bp to 50 kbp. Even at low concentrations the gel produced is very firm.

* availibility of sample size may be limited

49,00 €

order / kaufen

Final price excl. shipping costs³

verfügbar / avaílable
1 - 3 Tage Lieferzeit / 1-3 days delivery¹

Agarose usage

Method 1: Microwave oven

1. Pour buffer (approx. 90 % of final volume) into an appropriate flask that can accommodate up to four times the final gel volume and add a magnetic stir bar.
2. Put the flask with agarose onto a magnetic stirrer and slowly add agarose powder while stirring constantly to avoid clotting.
3. Remove flask magnetic stir bar.
4. Add remaining buffer up to the desired final volume.
5. Weigh and record the weight of of agarose and all components the flask prior to heating. Heat for 1 – 2 minutes in a microwave oven (600 Watt). Gently swirl the flask to mix the solution. Warning: Due to
microwave heating, there may be a delay in the liquid boiling!
6. Using the microwave oven, heat in short bursts of 5 – 10 seconds or until the solution withj agarose is boiling, with breaks of 10 – 15 seconds between heating phases to disperse bubbles by gently swirling the flask. Beware of hot glass ware and liquid. Continue until the agarose is completely dissolved.
7. Again, weigh the flask and top up lost volume with warm, deionized water. Gently swirl the flask to ensure complete mixing of agarose and all components.
8. Let the solution cool down at room temperature for 15 – 20 minutes or until a gel temperature of 50 – 60 °C is reached.

Method 2: Simmering water bath

1. see method 1 (agarose in micro wave oven): steps 1 – 2 and 4
2. Weigh and record the weight of the flask prior to heating. Heat agarose suspension up in a simmering water bath with constant stirring.
3. Leave the flask of agarose and all components bath in the water for further 15 – 20 minutes, or until the agarose is completely dissolved.
4. Switch off the magnetic stirrer and leave the flask with agarose in the bath for further 15 minutes.
5. Again, weigh the flask and top up lost volume with warm, deionized water. Gently swirl the flask to ensure complete mixing of agarose and all components.
6. Let the solution of agarose cool down at room temperature for 15 – 20 minutes or until a gel temperature of 50 – 60 °C is reached.

Storage: can be stored at room temperature for more than 4 years

Agarose free sample request

Dear customer, GeneON likes to send free samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .

Deutsche Beschreibung

Agarose für die Gelelektrophorese

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MSDS - Agarose for Post-PCR Analysis

Agarose from Hispanagar is a standard agarose for the separation of DNA in the size range between 50bp and 50kbp. It is suitable for all analytical and preparative electrophoresis of nucleic acids in routine gel electrophoresis. Depending on the concentration of Agarose LE used, the size range of nucleic acid separation will vary between 50bp and 20kbp. The low EEO makes this useful for a broad range of applications: PCR product analysis, restriction enzyme digest analysis, separation of RNA before blotting, etc

MSDS Agarose.pdf

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