Restriction Endonucleases

 

Aat I (Aat I)

Source: An E.coli strain, that carries the cloned Aat II gene from Acetobacter aceti.

GACGT↑C - C↓TGCAG

 

Abs I (Abs I)

Source: Arthrobacter species 7M06

CC↑TCGAGG / GGAGCT↓CC

 

AccBS I (BsrB I)

Source: Acinetobacter calcoaceticus BS

GAG↑CGG / CTC↓GCC

 

Acl I (Acl I)

Source: Acinetobacter calcoaceticus

AA↑CGTT - TTGC↓AA

 

Acs I (Apo I)

Source: Arthrobacter citreus

R↑AATTY - YTTAA↓R

 

Afe I (Eco47 III)

Source: Alcaligenes faecalis T2774

AGC↑GCT / TCG↓CGA

 

Ags I (Ags I)

Source: Agrococcus species 25

TTS↑AA / AA↓STT

 

Ahl I (Spe I)

Source: Alteromonas haloplanktis SP

A↑CTAGT / TGATC↓A

 

Ajn I (EcoR III)

Source: Acinetobacter johnsonii R2

↑CCWGG / GGWCC↓

 

Aox I (Aox I)

Source: Arthrobacter oxydans 25K

↑PuG(5mC)Py - Py(5mC)GPu↓

 

AspA2 I (Avr II)

Source: Arthrobacter species A2

C↑CTAGG - GGATC↓C

 

AsuNH I (Nhe I)

Source: Actinobacillus suis NH

G↑CTAGC - CGATC↓G

 

Bar I (Bar I)

Source: Bacillus sphaericus

↑(N)7GAAGNNNNNNTAC(N)12↑ -

↓(N)12CTTCNNNNNNATG(N)7↓

 

Bis I (Bis I)

Source: Bacillus subtilis T30

G(5mC)↑NGC - CGN↓(5mC)G

 

Bls I (Bls I)

Source: Bacillus simplex 23

PuPyN↑PuPy - PyPu↓NPyPu

 

Bmu I (Bfi I)

Source: Bacillus megaterium S87

ACTGGG(N)5↑ - ACTGGG(N)5↑

 

Bmt I (NheI)

Source: An E.coli strain that carries the cloned Bmt I gene from Bacillus megaterium S2

GCTAG↑C / C↓GATCG

 

Bpm I (Gsu I)

Source: Bacillus pumilus

CTGGAG(N)16↑ - GACCTC(N)14↓

 

Bpu10 I (Bpu10 I)

Source: An E.coli strain, that carries recombinant plasmids

CC↑TNAGC - GGANT↓CG

 

Bse1 I (Bsr I)

Source: Bacillus stearothermophilus 1

ACTGGN↑ - TGAC↓CN

 

BseP I (BspP I)

Source: Bacillus stearothermophilus P

G↑CGCGC - CGCGC↓G

 

BslF I (Fin I)

Source: Bacillus stearothermophilus Fl

GGGAC(N)10↑ - CCCTG(N)14↓

 

Bsp1720 I (Esp I)

Source: Bacillus species 1720

GC↑TNAGC - CGANT↓CG

 

Bsp19 I (Nco I)

Source: Bacillus species 19

C↑CATGG - GGTAC↓C

 

BspAC I (Aci I)

Source: Bacillus species AC

C↑CGC - GGC↓G

 

BssNA I (Sna I)

Source: Bacillus stearothermophilus NA

GTA↑TAC - CAT↓ATG

 

Bst4C I (Tsp4C I)

Source: Bacillus stearothermophilus 4C

ACN↑GT - TG↓NCA

 

BstAP I (ApaB I)

Source: Bacillus stearothermophilus AP

GCANNNN↑NTGC - CGTN↓NNNNACG

 

BstAU I (Bsp1407 I)

Source: Bacillus stearothermophillus AU

T↑GTACA - ACATG↓T

 

BstBA I (BsaA I)

Source: Bacillus stearothermophilus BA

YAC↑GTR - RTG↓CAY

 

BstC8 I (Cac8 I)

Source: Bacillus stearothermophilus C8

GCN↑NGC - CGN↓NCG

 

BstDS I (Dsa I)

Source: Bacillus stearothermophilus DS

C↑CRYGG - GGYRC↓C

 

BstKT I (Mbo I)

Source: Bacillus stearothermophilus KT

GAT↑C - C↓TAG

 

BstMA I (BsmA I)

Source: Bacillus stearothermophilus MA

GTCTC(N)1↑ - CAGAG(N)5↓

 

BstMW I (Mwo I)

Source: Bacillus stearothermophilus MW

GCNNNNN↑NNGC - CGNN↓NNNNNCG

 

BstPA I (PshA I)

Source: Bacillus stearothermophilus PA

GACNN↑NNGTC - CTGNN↓NNCAG

 

BstSF I (Sfe I)

Source: Bacillus stearothermophilus SF

C↑TRYAG - GAYRT↓C

 

BstSL I (BseS I)

Source: Bacillus stearothermophilus S

GKGCM↑C - C↓MCGKG

 

BstV1 I (Bbv I)

Source: Bacillus stearothermophilus V1

GCAGC(N)8↑ - CGTCG(N)12↓

 

Bsu I (BciV I)

Source: Bacillus sphaericus

GTATCC(N)6↑ - CATAGG(N)5↓

 

Cci I (BspH I)

Source: Curtobacterium citreus

T↑CATGA - AGTAC↓T

 

DseD I (Drd I)

Source: Deinococcus species D2

GACNNNN↑NNGTC - CTGNN↓NNNNCAG

 

Fal I (Fal I)

Source: Flavobacterium aquatile Ob10

↑(N)8AAGNNNNNCTT(N)13↑ - ↓(N)13TTCNNNNNGAA(N)8↓

 

Fat I (Nla III)

Source: An E.coli strain that carries the cloned Fat I gene from Flavobacterium aquatile NL3

↑CATG - GTAC↓

 

Fsp4H I (Fnu4H I)

Source: An E.coli strain that carries the cloned Fsp4H I gene from Flavobacterium species 4H

GC↑NGC - CGN↓CG

 

Fai I (Fai I)

Flavobacterium aquatile B15

YA↑TR - RT↓AY

 

Gla I (Gla I)

Source: Glacial ice bacterium GL29

Pu(5mC)↑GPy - PyG↓(5mC)Pu

 

Glu I (Glu I)

Source: Glacial ice bacterium GL24

G(5mC)↑NG(5mC) - (5mC)GN↓(5mC)G

 

Gsa I (BseY I)

Source: Geobacillus stearothermophilus Y

CCCAG↑C - G↓GGTCG

 

Kro I (Kro I)

Source: Kocurea rosea 307

G↓C(5mC)GGC - CGG(5mC)C↑G

 

Lmn I (Lmn I)

Source: Lysinibacillus manganicus An22

GCTCCN↑ - CGAG↓GN

 

Mfe I (Mfe I)

Source: An E.coli strain, that carries the cloned gene Mfe I from Mycoplasma fermentans.

C↑AATTG - GTTAA↓C

 

Mhl I (Sdu I)

Source: Micrococcus halobius SD

GDGCH↑C - C↓HCGDG

 

Mnl I (Mnl I)

Source An E.coli strain, that carries the cloned gene Mnl I from Moraxella

CCTC(N)7↑ - GGAG(N)

 

MspR9 I (ScrF I)

Source: Micrococcus species

CC↑NGG - GGN↓CC

 

Mte I (Mte I)

Source: Microbacterium testaceum 17B

G(5mC)G(5mC)↑NG(5mC)G(5mC) -

(5mC)G(5mC)GN↓(5mC)G(5mC)G

 

Pcs I (Pcs I)

Paracoccus carotinifaciens 3K

(5mC)GNNNNN↑NN(5mC)G -

G(5mC)NN↓NNNNNG(5mC)

 

Pci I (BspLU11 I)

Source: An E.coli strain that carries the cloned Pci I gene from Planococcus citreus SE-F45

A↑CATGT - TGTAC↓A

 

PciS I (Sap I)

Source: Planococcus citreus S

GCTCTTCN↑ - CGAGAAG(N)4↓

 

Pct I (Bsm I)

Source: Planococcus citreus SM

GAATGCN↑ - CTTAC↓GN

 

Pkr I (Pkr I)

Planomicrobium koreense 78k

GCN↑GC - CG↓NCG

 

Psi I (Psi I)

Source: Pseudomonas species-SE-G49

TTA↑TAA - AAT↓ATT

 

PspE I (BstE II)

Source: Pseudomonas species E

G↑GTNACC - CCANTG↓G

PspL I (Spl I)

Source: Pseudomonas species L

C↑GTACG - GCATG↓C

 

PspN4 I (Nla IV)

Source: Pseudomonas species N4

GGN↑NCC - CCN↓NGG

 

PspX I (PspX I)

Source: An E.coli strain that carries the cloned PspX I gene from Pseudomonas species A1-1

VC↑TCGAGB - BGAGCT↓CV

 

Psr I (Psr I)

Source: Pseudomanas stutzeri N2

↑(N)7GAACNNNNNNTAC(N)12↑ -

↓(N)12CTTGNNNNNNATG(N)7↓

 

PstN I (AlwN I)

Source: Pseudomonas stutzeri 217

CAGNNN↑CTG - GTC↓NNNGAC

 

Rig I (Fse I)

Source: Rhizobium yangligense

GGCCGG↑CC - CC↓GGCCGG 

 

Rsr2 I (Rsr II)

Source: Rhodobacter sphaeroides 12

CG↑GWCCG - GCCWG↓GC

 

Set I (Set I)

Source: Streptomyces werraensis 37

ASST↑ - ↓TSSA

 

SmiM I (MsI I)

Source: Sphingobacterium mizutae M

CAYNN↑NNRTG - GTRNN↓NNYAC

 

Sph I (SphI)

Source: An E.coli strain that carries the cloned Sph I gene from Streptomyces phaeochromogenes.

GCATG↑C - C↓GTACG

 

Xma I (Xma I)

Source: An E.coli strain, that carries the cloned gene XmaI from Xanthomonas malvacearum.

C↑CCGGG - GGGCC↓C

 

Zra I (Aat II)

Source: Zoogloea ramigera 11

GAC↑GTC - CTG↓CAG

 

Zsp2 I (Nsi I)

Source: Zoogloea species 2

ATGCA↑T - T↓ACGTA