Bovine Serum Albumin (BSA) is supplied with some restriction enzymes to prevent adhesion of the enzyme to reaction tubes and pipette surfaces. BSA also stabilizes some proteins during incubation.
The source of purified BSA is a molecular biology grade, protease free, fraction V starting material. This material is then processed to remove nucleases.
Concentration: 10 mg/ml (or other concentration on request)
Storage Buffer: dest. water or
in NEB-Buffer: 20 mM KPO4, 50 mM NaCl, 0.1 mM EDTA, 5% Glycerol, pH 7.0 (25°C)
Storage Temperature: -20°C
A 16 hour incubation is performed with ΦX174 DNA- HaeIII Digest. This assay checks for endonuclease, exonuclease and binding protein contamination. 16 hour incubation is performed with ΦX174 RFI
DNA, as sensitive assay for nonspecific endonuclease and 1 hour incubation with a RNA ladder is performed. Produced in ISO-certificated Company.
Transport: Ambient or with blue ice
- It is do not recommend using this preparation of BSA as a standard for
quantitation purposes or size determination.
- The BSA should be diluted 1:100 for standard reactions. At this level the
buffer from the BSA have a very small effect, only.