- In situ suppression (CISS) hybridizations
- Hybridization to micro-arrays
- Other In situ hybridizations
- Filter hybridizations
COT I Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements.
The product is prepared from male human placental DNA, exclusively.
The COT I DNA fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS), such as SINEs (small interspersed repetitive
elements, e.g., Alu elements) and LINEs (large interspersed repetitive elements, e.g., L1 elements),are distributed ubiquitously throughout the genome.
Concentration: > 1,1 mg/ml; Solution in 10 mM Tris-HCl, 1 mM EDTA, pH 7.4
The COT I DNA is available in concentration greater than 10,5 mg/ml.
- Average fragments size: 50-300 bp
- A260/A280 ration: about 1.70
- Amount of genomic (non-repetitive DNA): less than 5%
- COT I DNA and the raw material is tested for the absence of HIV1,2 RNA, HCV RNA, HBV DNA
Transportation: on blue ice
Storage: at -20°C for more than 12 months