The Kit contains two hydrolytic enzymes, recombinant Shrimp Alkaline Phosphatase (rSAP) and Exonuclease I (Exo I).
The combination of these enzymes ensures complete dephosphorylation of dNTPs and degradation of residual primers, enabling downstream applications, such as sequencing, genotyping, cloning or SNP analysis.
The reagents are active in commonly used PCR buffers and eliminates the need for additional buffer exchange.
Content: SAP-Exo Mix (red cap)
Shipping: shipped on blue ice
Storage Conditions: store at -20°C
Notes: avoid multiple freeze / thaw cycles
Shelf Life: at least 18 months
SAP-Exo Kit is a quick, easy and reliable enzymatic cleanup reagent for PCR product clean up.
• Removes excess primers and dNTPs
• Fast 20 minute protocol
• Add directly to PCR product
• 100% Sample Recovery
• Scalable for different reaction sizes
• No interference on downstream applications
• Easy to automate
-Add 2µl of SAP-Exo mix directly to 5µl PCR product (or scale up, i.g. 4µl
SAP-Exo mix to 10µl PCR product) and mix well
-Incubate for 10 min at 37°C
-Inactivate for 10 min at 80°C
PCR products are ready for downstream applications without any need for further processing.
Free shipping to the following countries: Afghanistan, Angola, Armenia, Austria, Azerbaijan, Bangladesh, Belgium, Bulgaria, Burkina Faso, Burundi , Algeria, Benin, Bhutan, Botswana, Brunei, Cambodia, Cameroon, Canada, Cape Verde, Central African Republic, Chad, China, Comoros, Congo - Brazzaville, Congo - Kinshasa, Côte d’Ivoire, Croatia, Czech Republic, Denmark, Djibouti, Equatorial Guinea, Eritrea, Estonia, Ethiopia, Finland, France, Gabon, Gambia, Germany, Ghana, Greece, Guinea-Bissau, Hong Kong SAR China, Hungary, India, Indonesia, Italy, Japan, Kazakhstan, Kenya, Kyrgyzstan, Laos, Lesotho, Liberia, Liechtenstein, Macau SAR China, Madagascar, Malawi, Malaysia, Maldives, Mali, Mauritania, Mauritius, Mayotte, Mongolia, Morocco, Mozambique, Myanmar [Burma], Namibia, Nepal, Netherlands, Niger, Nigeria, Northern Mariana Islands, North Korea, Norway, Pakistan, Philippines, Poland, Portugal, Réunion, Rwanda, São Tomé and Príncipe, Senegal, Seychelles, Sierra Leone, Singapore, Somalia, South Africa, South Korea, Spain, Sri Lanka, Sudan, Suriname, Swaziland, Sweden, Switzerland, Taiwan, Tajikistan, Tanzania, Thailand, Timor-Leste, Togo, Tunisia, Turkmenistan, Uganda, United Kingdom, United States, Uzbekistan, Vietnam, Western Sahara, Zambia, Zimbabwe
Dear customer, GeneON likes to send free samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .
Overview PCR Master Mixe:
BioStar 2 PCR mastermix with SYBRGreen blue dye, no rox
BioStar 3 PCR mastermix with SYBRGreen 60 nm LOW-ROX
BioStar 4 PCR mastermix with SYBRGreen 900 nm HIGH-ROX
BioStar 5 PCR mastermix
BioStar 6 PCR mastermix
for probes 60 mM LOW-ROX
BioStar 7 PCR mastermix
for probes 900 mM HIGH-ROX