Mutanolysin (EC 126.96.36.199) (N-acetylmuramidase) is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1->4)-N-acetylglucosamine linkage of the bacterial cell wall polymer
peptidoglycanpolysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall structures aboundant in many gram-positive bacteria.
Recombinant mutanolysin effectively lyses particularly problematic bacteria, like:
Streptococcus, Lactobacillus, Lactococcus, Listeria.
Recombinant Mutanolysin and lysozyme activity is synergistic. Using Mutanolysin and lysozyme mixture leads to increased yield of bacteria
One unit will produce a ∆A600 nm of 0.01 per minute at 50 mM MES
pH 6.0, 1 mM MgCl2 at 37 °C in a 1 ml volume using suspension of
Streptococcus faecalis cell wall as substrate.
Activity: > 3000-4000 U/mg
Concentration: Powder (to obtain concentration 10 U/µl dissolve the whole content of vial of mutanolysin lyophilisate in 1000 µl of Mutanolysin
Storage buffer (not included)
20 mM MES pH 6.2, 50 mM NaCl, 50% glycerol (v/v). 1 ml / 10000 U
Shipping: shipped on blue ice
Storage Conditions: store at -20°C
Notes: avoid multiple freeze / thaw cycles
Shelf Life: at least 18 months