DAPI - 4',6-Diamidino-2-phenylindole dihydrochloride

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels.

It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex.

Study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. (Molecular Biology Grade)

Synonym	4',6-Diamidino-2-phenylindole dihydrochloride
state of matter	Solid
Melting point	approx. 330°C
Formula	C₁₆H₁₅N₅ · 2HCl
M	350.25 g/mol
CAS-No.:	28718-90-3

EC-No.:	249-186-7
Storage:	2-8°C
	protected from light
LGK:	10 - 13
WGK:	1
Specification
DNases/RNases/Proteases	not detectable
Assay (HPLC)	min. 98 %
Solubility (1 %; H₂O)	clear
UV spectrum	complies to standard

Stability tests / Quality control / Comparison

DAPI

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Deutsche Beschreibung

Datasheet

Material Safety Datasheet

References / Protocols / Notes / Recomendations / Tests

(1)A simple cytochemical technique for demonstration of DNA in celles infected with mycoplasmas and viruses.
(Russel, W.C. et al. (1975) Nature 253, 461-462)
(2)Interaction of DAPI with synthetic polynucleotides. (Kapuscinski, J. & Szer, W. (1979) Nucleic Acids Res. 6,
3519-3534)
(3)Binding of DAPI to GC and Mixed Sequences in DNA: Intercalation of a Classical Groove-Binding Molecule.
(Wilson, W.D. et al. (1989) J. Am. Chem. Soc. 111, 5008-5010)
(4)DAPI Staining of Fixed Cells for High-Resolution Flow Cytometry of Nuclear DNA. (Otto, F. (1990) Methods
Cell Biol. 33, 105-110)
(5)Reverse Fluorescent Chromosome Banding with Chromomycin and DAPI. (Schweizer, D. (1976)
Chromosoma 58, 307-324)
(6)Quantitative Fluorescent Analysis of Different Conformational Forms of DNA Bound to the Dye DAPI, and
Separated by Gel Electrophoresis. (Naimski, P. et al. (1980) Anal. Biochem. 106, 471-475)
(7)Spectrofluorometry of Dyes with DNAs of Different Base Composition and Conformation. (Daxhelet, G.A. et
al. (1989) Anal. Biochem. 179, 401-403)
(8)Detection of Mycoplasma Infection of Mammalian Cells. (Xia, H. et al. (1997) BioTechniques 22, 934-936)

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DAPI - 4',6-Diamidino-2-phenylindole dihydrochloride

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels.

It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex.

Study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. (Molecular Biology Grade)