The Maximo-DNA Cycle Sequencing Kit provides a powerful tool to derive rapidly DNA and gene sequence information as required in a multitude of
molecular biological and biotechnological applications.
The performance of the kit is based on a enhanced Taq polymerase showing an equal capability of incorporating ddNTPs and dNTPs. As a result the
Maximo-Cycle Sequencing-Kit offers uniform and easy to read sequence band patterns at lowest background.
An absolutely minimal band compression of GC-rich DNA regions is realized by optimally balanced termination mixtures containing 7-deaza-dGTP.
The reaction chemistry of the kit is optimized for automated DNA sequencers and requires labelled primers with fuorescent dyes.
Terminate solution A (blue cap): dNTP mix containing ddATP
Terminate solution C (blue cap): dNTP mix containing ddCTP
Terminate solution G (blue cap): dNTP mix containing ddGTP
Terminate solution T (blue cap): dNTP mix containing ddTTP
Cycle sequencing Polymerase (red cap): 4 Units/µl
Cycle sequencing Buffer (green cap): 10 fold
PCR-grade water (white cap)
Stopsolution (purple cap): 95 % formamide containing EDTA, bromophenol
blue, and xylene cyanolFF
Shipping: shipped on blue ice
Storage Conditions: store at -20°C
Note: avoid multiple freeze / thaw cycles
Shelf Life: 18 months
DNA cycle sequencing is a core technique in molecular biology allowing analysis of fmol-quantities DNA template. The enzymatic dideoxy chain termination method of Sanger relies on the linear
ampliﬁcation of a single-stranded template DNA using a single primer and thermostable polymerase. The synthesis of the complementary DNA strand starts at the speciﬁc priming site and ends with
the incorporation of a chain-terminating dideoxynucleotide triphosphate (ddNTP). This generates a multitude of fragments terminated within the desired length of the sequence. By using the four
different ddNTPs in four separate reaction vials, a set of extended primer strands terminated at each A, C, G, and T are obtained. When these fragments are separated on a suitable gel matrix the
sequence information can be read from the order of the bands.
The kit is optimized for cycle sequencing using ﬂuorescent-labelled primers. The required 5’-end ﬂuorescent label of the primer depends on the optical set-up of the used sequencing machine.
Primers should typically be 20-25 nucleotides in length with a content of 50-60 % G+C. They should be checked to avoid forming of internal duplexes or mispriming to other sites of the template.
Minimize the exposure of ﬂuorenscent- labelled primers to light.
produced in ISO-certificated company