Array CGH, Cell Analysis, Cellular Imaging, ChIP-on-Chip, Chromatin Biology, Fluorescence In Situ Hybridization, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, In Situ
Hybridization (ISH), Microarray Analysis, RNAi, Epigenetics and Non-Coding RNA Research
COT I Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements. The
product is similar to Invitrogen #15279.
The product is prepared from male human placental DNA, exclusively.
The Cot-I DNA fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS), such as SINEs (small interspersed repetitive
elements, e.g., Alu elements) and LINEs (large interspersed repetitive elements, e.g., L1 elements),are distributed ubiquitously throughout the genome.
Concentration: 1,1 mg/ml; Solution in 10 mM Tris-HCl, 1 mM EDTA, pH 7.4
Transportation: on blue ice
Storage: at -20°C for more than 12 months
value Test results
Appearance clear, colourless
Concentration (abs. 260 nm; 0.9 –1.2
mg/ml 1,11 mg/ml
in 50mM NaOH)
Non-COT 1 DNA < 5%
Y-Chromosome obtained exclusively from male
Determination of quotient A260/A280: 1.6 –
HIV1,2, HCV and HBV RNA/DNA not detectable
Gel electrophoretic separation in 1.2 % agarose
gel 2.0,3.0 µg without RE cleavage middle chain
length: 50 – 300
Treated by phenol in the process of
10mM TrisHCl, 1mM EDTA, pH 7,4