Array CGH, Cell Analysis, Cellular Imaging, ChIP-on-Chip, Chromatin Biology, Fluorescence In Situ Hybridization, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, In Situ Hybridization (ISH), Microarray Analysis, RNAi, Epigenetics and Non-Coding RNA Research
COT I Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements. The product is similar to Invitrogen #15279.
The product is prepared from male human placental DNA, exclusively.
The Cot-I DNA fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS), such as SINEs (small interspersed repetitive elements, e.g., Alu elements) and LINEs (large interspersed repetitive elements, e.g., L1 elements),are distributed ubiquitously throughout the genome.
Concentration: 1,1 mg/ml; Solution in 10 mM Tris-HCl, 1 mM EDTA, pH 7.4
Transportation: on blue ice
Storage: at -20°C for more than 12 months
Concentration (abs. 260 nm; 0.9 –1.2 mg/ml 1,11 mg/ml
in 50mM NaOH)
Ratio C(OD260/)C(Hoechst) ≤1,5 1,45
Non-COT 1 DNA < 5% w/w corresponds
Y-Chromosome obtained exclusively from male
human placenta corresponds
Determination of quotient A260/A280: 1.6 – 2.0 1.82
HIV1,2, HCV and HBV RNA/DNA not detectable
( PCR/RT-PCR) corresponds
Gel electrophoretic separation in 1.2 % agarose
gel 2.0,3.0 µg without RE cleavage middle chain
length: 50 – 300 bp corresponds
Treated by phenol in the process of production corresponds
Buffer 10mM TrisHCl, 1mM EDTA, pH 7,4
Cot-1 DNA for: In situ hybridisation, micro-array, DNA array comparative genomic Hybridisation (CGH), and genetic analyzing
* availibility of sample size may be limited
Dear customer, GeneON likes to send free samples of Cot I DNA accurate to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .
Taqs: cot 1, Cot I
Cot I Humane DNA (acc. Fluoro)
Recommendation from a researcher:
For Southern blot hybridizations: add 50 μg of COT-1 human DNA (@ 10 μg/μL) to 50 μL of 20X SSC, 25 μL distilled water and 20 μL of a solution containing 0.1 M NaCl, 0.1 M Tris-HCl (pH 7.4). 0.01 M EDTA, and 1% SDS to the probe for each 25 to 500 ng of probe.
For in situ hybridizations: combine genomic probe with the proper amount of COT-1 human DNA such that the final concentration of COT-1 human DNA is 0.3 μg/μL for cosmid, plasmid, and lambda probes; or, at 1 μg/μL for Alu PCR probes.
Ethanol precipitate and resuspend in a half-volume of 100% formamide. Add a half-volume of 20% dextran sulfate in 2X SSC (prewarmed to 75 degrees C) and mix well. Denature mix by heating to 75 °C for 5 min. Incubate at 37 °C for at least 5 and up to 15 min.