COT I Human DNA acc. Fluoro.

Cot-1 DNA for: Epigenetics and Non-Coding RNA Research, Genotyping and Genomic Profiling

Applications:

Array CGH, Cell Analysis, Cellular Imaging, ChIP-on-Chip, Chromatin Biology, Fluorescence In Situ Hybridization, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, In Situ Hybridization (ISH), Microarray Analysis, RNAi, Epigenetics and Non-Coding RNA Research

 
Description:
COT I Human DNA is prepared from human placental DNA by shearing, denaturing, and reannealing under conditions that enrich these repetitive elements. The product is similar to Invitrogen #15279.
The product is prepared from male human placental DNA, exclusively.
The Cot-I DNA fraction of human genomic DNA consists largely of rapidly annealing repetitive elements. These interspersed repetitive sequences (IRS), such as SINEs (small interspersed repetitive elements, e.g., Alu elements) and LINEs (large interspersed repetitive elements, e.g., L1 elements),are distributed ubiquitously throughout the genome.

Concentration: 1,1 mg/ml; Solution in 10 mM Tris-HCl, 1 mM EDTA, pH 7.4

Quality control:
see below

Transportation: on blue ice

Storage: at -20°C for more than 12 months

 

Stability tests / Last Lot QC result (January 2018)

Parameter Range                                                          value Test results

Appearance clear, colourless solution                                                            corresponds

 

Concentration (abs. 260 nm; 0.9 –1.2 mg/ml                                  1,11 mg/ml

in 50mM NaOH)

 

Ratio C(OD260/)C(Hoechst) ≤1,5                                                      1,45

 

Non-COT 1 DNA < 5% w/w                                                                   corresponds

 

Y-Chromosome obtained exclusively from male

human placenta                                                                                     corresponds

 

 Determination of quotient A260/A280: 1.6 – 2.0                             1.82

 

HIV1,2, HCV and HBV RNA/DNA not detectable

( PCR/RT-PCR)                                                                                      corresponds

 

Gel electrophoretic separation in 1.2 % agarose

gel 2.0,3.0 µg without RE cleavage middle chain

length: 50 – 300 bp                                                                             corresponds

 

Treated by phenol in the process of production                         corresponds

 

Buffer                                                         10mM TrisHCl, 1mM EDTA, pH 7,4

 

Humane COT I DNA accurate Fluorometrie, COT 1,
# 31002 COT I Human DNA 500µg

Cot-1 DNA for: In situ hybridisation, micro-array, DNA array comparative genomic Hybridisation (CGH), and genetic analyzing

 

* availibility of sample size may be limited

Cot I DNA accurate
209,00 € 2
165,00 €
order / kaufen
  • verfügbar / avaílable
  • 1 - 3 Tage Lieferzeit / 1-3 days delivery1

Free sample request Cot 1 DNA acc. Flouro.

Dear customer, GeneON likes to send free samples of Cot I DNA accurate to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .

 

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Ultra pure Nucleotides

Taqs: cot 1, Cot I


Deutsche Beschreibung

Cot I Humane DNA (acc. Fluoro)

Datasheet Cot I human DNA Acc. Fluoro

Material Safety Datasheet

Material Safety Datasheet Cot I Human DNA
COT I MSDS.pdf
Adobe Acrobat Document 83.6 KB

References / Protocols / Notes / Recomendations / Tests

Recommendation from a researcher:


For Southern blot hybridizations: add 50 μg of COT-1 human DNA (@ 10 μg/μL) to 50 μL of 20X SSC, 25 μL distilled water and 20 μL of a solution containing 0.1 M NaCl, 0.1 M Tris-HCl (pH 7.4). 0.01 M EDTA, and 1% SDS to the probe for each 25 to 500 ng of probe.

For in situ hybridizations: combine genomic probe with the proper amount of COT-1 human DNA such that the final concentration of COT-1 human DNA is 0.3 μg/μL for cosmid, plasmid, and lambda probes; or, at 1 μg/μL for Alu PCR probes.
Ethanol precipitate and resuspend in a half-volume of 100% formamide. Add a half-volume of 20% dextran sulfate in 2X SSC (prewarmed to 75 degrees C) and mix well. Denature mix by heating to 75 °C for 5 min. Incubate at 37 °C for at least 5 and up to 15 min.

Interesting links:

Bao Paul; Frutos Anthony G; Greef Charles; Lahiri Joydeep; Muller Uwe; Peterson Todd C; Warden Laurence; Xie Xinying; 
Anal Chem (2002) 74:1792-1799
Product usage: Superscript II RT was used to make labeled cDNA from a human polyA RNA sample (42C incubation temp). Cy3 or Cy5 labeled dCTP was used for these experiments. Remaining RNA was degraded by using a combination of RNase H (Invitrogen) and RNase A (USB). Array hybridization was per......

Tang Y M; Wo Y Y; Stewart J; Hawkins A L; Griffin C A; Sutter T R; Greenlee W F; 
J Biol Chem (1996) 271:28324-28354
Product usage: Cultures of the human squamous cell carcinoma line SCC12(c12c2) were plated at 2.5 × 106 to 5 × 106 cells/60-mm dish and grown to 70-90% confluence before transfection. The transient transfection was performed by a lipofection method in a serum-free medium with 2.5 µg of each pla......

 

 

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