Proteinase K: Powder and customized solutions
Proteinase K and the solution is used for protease digestion during DNA and RNA preparation. Proteinase K from GeneON is very active and stable, even at room temperature. The enzyme is used as component in Diagnostics Kits or in DNA/RNA purification systems.
Enzyme for digestion of proteins
Description:
Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The Protein with a molecular weight 28.900 kD cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinase K is not inactivated by chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12.5).
Activity: > 30 units/mg protein (haemoglobin, pH 7.5, 37°C)
Unit definition:
Unit definition One unit is the amount of enzyme which releases at 37°C in 1 min as many folin-positive amino acids and peptides from haemoglobin as 1 μmol of tyrosine.
Features:
The protease or - its solution is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine
proteases and is strongly inhibited by PMSF.
Usage:
In presence of 0.5 – 1 % SDS Proteinase inactivates DNases and RNases in eukaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of
intact highly-molecular nucleic acids.
Quality:
- purified by chromatography and lyophilised
- RNases: not detectable
- DNases: not detectable
- Exonucleases: not detectable
Storage:
4 °C or -20 °C for at least 24 months, shipment at ambient temperature
Storage buffer: As an option GeneON offers: 50 mM Tris HCl (pH 8.0), 1 mM CaCl2, 50 % Glycerin (other buffers or other concentrations on request)
Custom aliquoting service
GeneON provides customized amounts of Proteinase K Powder and storage buffer on request. We precisely dispense Prot.-K aliquots (liquid/lyophilized) that will fit the format of your choice.
Free sample request for Proteinase
Dear customer, GeneON likes to send free samples of to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .
Deutsche Beschreibung: Proteinase K
Related products
Proteinase K
Proteinase K as powder
GeneON offers several sizes and sets of Proteinase K:
Proteinase K as powder: 4x25 mg / 200 mg / 500 mg / 1000 mg, 50 g or 100 g
Proteinase K powder and Storagebuffer separatly: 1x125 mg + 6,2 ml buffer (50 mM Tris HCl (pH 8.0), 1 mM CaCl2, 50 % Glycerin)
Proteinase K powder: as bulk or in customized packsizes
Final price excl. shipping costs3
- verfügbar / avaílable
- 1 - 3 Tage Lieferzeit / 1-3 days delivery1
Proteinase K as solution
GeneON offers several sizes and sets of Proteinase K:
Proteinase K as solution: 20 mg/ml (50 mM Tris HCl (pH 8.0), 1 mM CaCl2, 50 % Glycerin)
Proteinase K powder and Storagebuffer separatly: 1x125 mg + 6,2 ml buffer
Proteinase K solution: as bulk or in customized packsizes and concentration
Final price excl. shipping costs3
- verfügbar / avaílable
- 1 - 3 Tage Lieferzeit / 1-3 days delivery1
Datasheet Proteinase K
Stability Test for Proteinase K
References:
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GeneOn GmbH
Paracelsusstrasse 10
67071 Ludwigshafen am Rhein
Deutschland / Germany
Phone: +49-621-5720 864
Fax: +49-621-5724 462
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