Proteinase K: Powder and customized solutions

Proteinase K and the solution is used for protease digestion during DNA and RNA preparation. Proteinase K from GeneON is very active and very stable even at room temperature. As component in Diagnostics Kits or in DNA/RNA purification systems.

Application:
Digestion of proteins

Description:
Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The Protein with a molecular weight 28.900 kD cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinase K is not inactivated by chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12.5).

Activity: > 30 units/mg protein (hemoglobin, pH 7.5, 37°C)

Unit definition:
Unit definition One unit is the amount of enzyme which releases at 37°C in 1 min as many folin-positive amino acids and peptides from hemoglobin as 1 μmol of tyrosine.

Features:
Proteinase K or - solution is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.

Usage:
In presence of 0.5 – 1 % SDS Proteinase inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.

Quality:
- purified by chromatography and lyophilised
- RNases: not detectable
- DNases: not detectable
- Exonucleases: not detectable

Storage:
4 °C or -20 °C for at least 24 months, shipment at ambient temperature

Storagebuffer: 50 mM Tris HCl (pH 8.0), 1 mM CaCl2, 50 % Gycerin (other buffer or other concentrations on request)

Proteinase K as powder or solution — Proteinase K as customized solutions

GeneON provides all customized amounts of Proteinase K Powder and storage buffer on request. Even with your label...

Proteinase K powder and solution

Proteinase K isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. Proteinase K from GeneON is very active and stable

* availibility of sample size may be limited

65,00 € ²

58,00 €

order / kaufen

Final price excl. shipping costs³

verfügbar / avaílable
1 - 3 Tage Lieferzeit / 1-3 days delivery¹

Free sample request for Proteinase

Dear customer, GeneON likes to send free samples of to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .

Deutsche Beschreibung: Proteinase K

Proteinase K

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Proteinase K or Proteinase K solution is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.

Proteinase K +.pdf

Adobe Acrobat Document 87.0 KB

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Material Safety Datasheet

MSDS Proteinase K Powder.pdf

Adobe Acrobat Document 125.6 KB

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Stability Test for Proteinase K

Proteinase K stability test at different temperatures, logistic issue for Proteinase k

References:

Betzel, C., Three Dimensional Structure of Proteinase K at 0.15 nm Resolution. Eur. J. Biochem., 178, 155-171 (1988).
Ebeling, W., et al., Proteinase K from Tritirachium album Linder, Eur. J. Biochem., 47, 91 (1974).
Enzymes of Molecular Biology, vol. 16, Burrell, M.M., ed. Humana Press (Totowa, NJ: 1993), p. 307. Kraus, E., and Femfert, U., Proteinase K from the Mold Tritirachium album Limber, Specificity and Mode of Action. Z. Physiol. Chem., 357, 937 (1976).
Lizardi, P.M., and Engelberg, A., Rapid Isolation of RNA Using Proteinase and Sodium Perchlorate. Anal. Biochem., 98, 116 (1979).

¹ Applies to shipping within Germany. Information about shipping policies for other countries can be found here: Payment and Delivery Information
² limitierter Sonderpreis / Special Price limited
³ Nettopreise ohne Umsatzsteuer zzgl. Frachtkosten / All prices without VAT excl. freight costs. :: MUSTER FÜR DEUTSCHLAND WERDEN KOSTENFREI GELIEFERT - AUCH WENN IM SHOP EIN PREIS AUSGEWIESEN WIRD :::

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Proteinase K: Powder and customized solutions

Proteinase K and the solution is used for protease digestion during DNA and RNA preparation. Proteinase K from GeneON is very active and very stable even at room temperature. As component in Diagnostics Kits or in DNA/RNA purification systems.

GeneON provides all customized amounts of Proteinase K Powder and storage buffer on request. Even with your label...