Enzyme for digestion of proteins
Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The Protein with a molecular weight 28.900
kD cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinase K is not inactivated by chelating reagents such as EDTA or detergents such as SDS and
is active over a wide range of pH (4-12.5).
Activity: > 30 units/mg protein (haemoglobin, pH 7.5, 37°C)
Unit definition One unit is the amount of enzyme which releases at 37°C in 1 min as many folin-positive amino acids and
peptides from haemoglobin as 1 μmol of tyrosine.
The protease or - its solution is a highly active and stable protease with low cutting specificity. The enzyme belongs to the
group of subtilisine-related serine proteases and is strongly inhibited by PMSF.
In presence of 0.5 – 1 % SDS Proteinase inactivates DNases and RNases in eukaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of
intact highly-molecular nucleic acids.
- purified by chromatography and lyophilised
- RNases: not detectable
- DNases: not detectable
- Exonucleases: not detectable
Each batch of Proteinase K is tested for the presence of fungal and bacterial DNA. Moreover, the specific activity assay of
each batch of the enzyme is performed.
4 °C or -20 °C for at least 24 months, shipment at ambient temperature
Storage buffer: As an option GeneON offers: 50 mM Tris HCl (pH 8.0), 1 mM CaCl2, 50 % Glycerin (other buffers or other concentrations
GeneON offers several sizes and sets of Proteinase K:
Proteinase K as powder: 4x25 mg / 200 mg / 500 mg / 1000 mg, 50 g or 100 g
Proteinase K powder and Storagebuffer separatly: 1x125 mg + 6,2 ml buffer (50 mM Tris HCl (pH 8.0), 1 mM CaCl2, 50 % Glycerin)
Proteinase K powder: as bulk or in customized packsizes