Proteinase K: Powder and customized solutions

Features:
The protease or - its solution is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.

Usage:
In presence of 0.5 – 1 % SDS Proteinase inactivates DNases and RNases in eukaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.

Quality:
- purified by chromatography and lyophilised
- RNases: not detectable
- DNases: not detectable
- Exonucleases: not detectable

Each batch of Proteinase K is tested for the presence of fungal and bacterial DNA. Moreover, the specific activity assay of each batch of the enzyme is performed. 

Storage:
4 °C or -20 °C for at least 24 months, shipment at ambient temperature

Storage buffer: As an option GeneON offers: 50 mM Tris HCl (pH 8.0), 1 mM CaCl2, 50 % Glycerin (other buffers or other concentrations on request)

Determination of the the stability of Proteinase K during long-term storage at room temperature. The tested Lot was compared to the fresh production batch in 6 month time intervals. Assay conditions described below.

Lot: tested: P040917 control LOT: current production batch

Storage buffer for Proteinase K in solution:

20 mM Tris pH 7,5, 1 mM CaCl2, 0,02% sodium acid, 50% glycerol (v/v).

Test results

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Assay conditions

●      Temperature: 37 °C, A750

●      Reaction volume: 900 μl

●      Substrate Solution: 2% Haemoglobin with 6M Urea pH 7.5

●      Spectrophotometer: Eppendorf Biospectrometer