Proteinase K Powder / Proteinase K solutions
Proteinase K and the solution isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. Proteinase K and from GeneON is very active and very stable even at room temperature.
Digestion of proteins
Description:
Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The Protein with a molecular weight 28.900 kD cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinase K is not inactivated by chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12.5).
Activity: > 30 units/mg protein (hemoglobin, pH 7.5, 37°C)
Unit definition:
Unit definition One unit is the amount of enzyme which releases at 37°C in 1 min as many folin-positive amino acids and peptides from hemoglobin as 1 μmol of tyrosine.
Features:
Proteinase K or Proteinase K solution is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related
serine proteases and is strongly inhibited by PMSF.
Usage:
In presence of 0.5 – 1 % SDS Proteinase K inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of
intact highly-molecular nucleic acids.
Quality of Proteinase K:
- purified by chromatography and lyophilised
- RNases: not detectable
- DNases: not detectable
- Exonucleases: not detectable
Storage:
4 °C or -20 °C for at least 24 months, shipment at ambient temperature
GeneON provides all customized amounts of Proteinase K Powder and storage buffer on request. Even with your label...
Proteinase K isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. Proteinase K from GeneON is very active and stable
* availibility of sample size may be limited
- Available
Free sample request for Proteinase
Dear customer, GeneON likes to send free samples of Proteinase K to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .
Related products
Proteinase K
Datasheet Proteinase K
Material Safety Datasheet
Stability Test for Proteinase K
References:
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Phone: +49-621-5720 864
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info@geneon.net
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