Proteinase K: Powder and customized solutions

Features:
The protease or - its solution is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.

Usage:
In presence of 0.5 – 1 % SDS Proteinase inactivates DNases and RNases in eukaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.

Quality:
- purified by chromatography and lyophilised
- RNases: not detectable
- DNases: not detectable
- Exonucleases: not detectable

Each batch of Proteinase K is tested for the presence of fungal and bacterial DNA. Moreover, the specific activity assay of each batch of the enzyme is performed. 

Storage:
4 °C or -20 °C for at least 24 months, shipment at ambient temperature

Storage buffer: As an option GeneON offers: 50 mM Tris HCl (pH 8.0), 1 mM CaCl2, 50 % Glycerin (other buffers or other concentrations on request)

GeneON offers several sizes and sets of Proteinase K:

Proteinase K as powder: 4x25 mg / 200 mg / 500 mg / 1000 mg, 50 g or 100 g

Proteinase K powder and Storagebuffer separatly: 1x125 mg + 6,2 ml buffer (50 mM Tris HCl (pH 8.0), 1 mM CaCl2, 50 % Glycerin)

Proteinase K powder: as bulk or in customized packsizes

GeneON offers several sizes and sets of Proteinase K:

Proteinase K as solution: 20 mg/ml (50 mM Tris HCl (pH 8.0), 1 mM CaCl2, 50 % Glycerin)

Proteinase K powder and Storagebuffer separatly: 1x125 mg + 6,2 ml buffer

Proteinase K solution: as bulk or in customized packsizes and concentration