GREEN DNA-RNA-dye

High level sensitivity, substitute to ethidium bromide (20000x)

Description:

GREEN DNA-RNA dye is a newly developed fluorescent dye for the detection of nucleic acids. It is applicable for staining of dsDNA, ssDNA as well as RNA. Sensitivity is comparable to ethidium bromide. GREEN DNA-RNA is non-toxic and ecofriendly and thus an uncompromising alternative to ethidium bromide. For staining, GREEN DNA-RNA can directly be added to the gel. Alternatively, staining is possible after electrophoresis.

Features:

- Fluorescent dye for dsDNA, ssDNA and RNA

- 20000 x concentrated

- Supplied in water instead of DMSO

- Compatible with common ethidium bromide documentation systems

- Excitation with UV or blue light (excitation maxima 295 nm and 490 nm)

- Detection same as ethidium bromide (emission maximum 530 nm)

- Storage at 4 °C, light-protected

Protocol: please see manual for Pre - or Post-staining

Picture:
TAE Agarose gel: 1,5 %; 312 nM UV-Table, 590 nm filter.
Green bands: Green DNA-RNA-dye 1:20000
Red bands: Ethidiumbromide 0,5 µg/ml

Stability tests / Quality Tests

Note: It is highly recommended agarose gel to be ≤ 0.5 cm in thickness. TAE buffer system is preferred for higher sensitivity.

Detection: Detect bands under UV illumination or non-UV LED illuminators such as Blue Light LED illumination. (For UV illumination we recommend yellow or green gelatine- or cellophane filters for clearer bands.)

Shipment: at room temperature

Storage: at +4 °C for at least 12 months, protect from light

Disposal: GREEN DNA-RNA is not cassified as hazardous waste. It can be disposed as regular laboratory trash in accordance to the local regulations.

GREEN DNA-RNA-dye