ViSafe Red Gel Stain is designed to replace the highly toxic ethidium bromide (EtBr). Both red gel stain and EtBr have the same spectra, so the red gel stain able to replace the EtBr without
changing existing imaging system. The dye is confirmed by Ames test results that it is impenetrable to latex gloves and cell membranes. By using the suggested working concentrations in gel
staining, the red gel stain is proven unable to cross cell membranes; and it is noncytotoxic and nonmutagenic at working concentrations.
ViSafe Red Gel Stain, 10000X in H2O, can be diluted 10000X for precast gel protocol or 3000-3300X for post gel staining. One vial (0.5ml) of 10000X solution can be used for at least 100 minigels
either using precast method or poststaining method.
- The red gel stain is non cytotoxic & non mutagenic shown by Ames
- It has a higher sensitivity More sensitive compared to EtBr or Viva
SybrGreen Nucleic Acid Stain.
- Extremely stable at room temperature for long-term storage.
Stable to be microwaved or being heated. The working solution is stable at room temperature when kept in dark. Wide application Suitable to stain dsDNA, ssDNA and RNA.
It is suitable to use in agarose gel or polyacrylamide gel and compatible with downstream applications, such as gel recovery & cloning.
Easy staining protocols and easy precast gel staining & post-staining procedures.
Compatible with most imaging system Gel can be viewed with standard UV transilluminator, visible light gel reader, or other gel imaging system.
Shipment: @ ambient temperature
Storage: 4-8°C or -20°C
Precast Protocol for 1% Agarose Gel
1. Pour 1g of agarose powder and 100ml 1X TAE or 1X TBE into glass
2. Melt the agarose in microwave for 1-3 mins until the agarose is
3. Add 3-5µl red gel stain per 100ml gel and stir the gel solution to mix
Make sure the red gel stain is swirled and stirred well to mix with gel solution.
4. Pour the agarose solution onto gel plate and insert a comb.
5. Place newly poured gel at 4°C for 10-15 mins or stay at room
temperature for 20-30 mins, until it has completely solidified.
*Extra agarose containing the red gel stain can be kept in solid form at 4°C and can be remelted to cast more gels.
6. After the gel is ready, perform gel electrophoresis.
7. Visualize or image the gel directly under UV light or blue light after gel electrophoresis is done. Standard transilluminator or ethidium bromide filter can be used for gel imaging purpose.
*Dilute the stock solution into agarose gel solution at 1:10000.
*Since the red gel stain is thermally stable, the stock solution can be
added while the gel solution is still hot.
*The red gel stain can be pre-combined with agarose powder and gel
working solution followed by microwaving or other heating procedures.
- However, more dye might be necessary to be added for optimal signal.
- Agarose containing the red gel stain is not recommended to be stored in
molten form for more than a fewdays.