- labeling of DNA
- nick translation of DNA
- 3'-end labeling
- cDNA synthesis or primer extension reactions
- incorporation into DNA using: Reverse Transcriptase, Taq DNA Polymerase, Klenow Fragment
Biotin-11-dUTP (Biotin-11-2'-deoxyuridine-5'-triphosphate, tetralithium salt) compound for non-radioactive DNA labeling.
The number "11" is the number of carbon atoms in the backbone of linker between dUTP and biotin. The longer the linker is, the more effective interaction of biotin with avidin occurs. The length
of spacer "11" is optimal for most of applications.
Quality: more than 96% (IEC, TLC, NMR, UV)
Concentration: 1 mM
Storage buffer: 10 mM TrisHCl, pH 7.5, 1 mM EDTA
Shipping: with "blue ice"