Applications for Nukleosidtriphosphate solution as mix or set:

- all molecular biology applications 
- PCR/qPCR
- reverse transcription
- DNA labelling 
- DNA sequencing

ALL dNTP's are free of human or bacterial DNA

Description dNTP-solution-mix and dNTP-sets:
dNTP-sets from GeneON have PCR Grade and contains four separate tubes of dATP, dCTP, dGTP and dTTP supplied as aqueous solutions at pH 8.5. The dNTP-Mix contains an optimized mixture of dNTP`s with 10 mM of each nucleoside.

Quality control dNTP mix and set of Nukleosidtriphosphat:

- purity: purity test with RP-HPLC: greater 99 % (stable from lot-to-lot), 

- Amplification tests:
   Lambda DNA: 18 kb fragment, less than 20 pg template
   RT-PCR: Human DNA, 600 bp fragment, less than 20 pg template

- Free of bacterial - and human DNA

- Free of: DNase, RNase, Protease and no nicking activity

- produced in German factory (ISO-9001 certificated)

Purity of dNTPs is a big factor in PCR performance. A specification of ≥ 99.0 % dNTP is the market standard. But the purity is not the the most important point. The constitution of the remaining ≤ 1.0 % is crucial.

Common contaminants in dNTP solutions are:

- Deoxyribonucleotide diphosphates – dNDPs

- Ribonucleotides – NTPs

- Other dNTPs – e.g. deaminated or methylated dNTPs

- Other nucleosidic compounds

- Phosphates

Even trace quantities of other impurities can significantly affect performance, hence our aim is to minimize all traces.

You need 1 Liter of dNTP'S from one Batch? We are ready to provide for a reasonable price within shortest time.