The Direct PCR Complte Kit contains all components for fast extraction of DNA and the analysis by Realtime PCR or Standard PCR.
The Extraction buffer is not harmful or does not contain any irritation substances.
The optimal mixture of Extraction buffers and PCR-Enzyme mix guaranties a fast and repeatable result.
- Less than 20 minutes extraction procedure
- Simple extraction step in one 0,2 ml PCR-tube – no need for washing or
- PCR Enzyme Mix includes all components even coloured loading buffer
for post PCR procedure
Direct enzymatical extraction and storrage of DNA from many samples like:
- FFPE tissue
- Hair follicle
- Animal Tissue
- Direct PCR with Hot-Start-Enzyme mix
- Direct PCR-Product analysis on Agarose-Gel.
The Direct-PCR Kit contains:
- Extraction Buffer (EXT-buffer), 10000 µl
- Thermostable Enzyme Mix (ENZ-Mix), 500 µl
- Hot-Start PCR Mix with loading dye, 1300 µl
- Sterile Water 1500 µl
The components are enough for about 100 reactions (25µl/reaction)
Storage: -20°C for about 12 months
Transport: on blue ice
Additionally required (not included):
0,2 ml PCR Tubes, Thermoblock, Waterbath or Thermocycler, optional for swaps sample 1,5 ml Tubes,