The Direct PCR Complte Kit contains all components for fast extraction of DNA and the analysis by Realtime PCR or Standard PCR.
The Extraction buffer is not harmful or does not contain any irritation substances.
The optimal mixture of Extraction buffers and PCR-Enzyme mix guaranties a fast and repeatable result.
- Less than 20 minutes extraction procedure
- Simple extraction step in one 0,2 ml PCR-tube – no need for washing or
- PCR Enzyme Mix includes all components even coloured loading buffer
for post PCR procedure
Direct enzymatical extraction and storrage of DNA from many samples like:
- FFPE tissue
- Hair follicle
- Animal Tissue
- Direct PCR with Hot-Start-Enzyme mix
- Direct PCR-Product analysis on Agarose-Gel.
The Direct-PCR Kit contains:
- Extraction Buffer (EXT-buffer), 10000 µl
- Thermostable Enzyme Mix (ENZ-Mix), 500 µl
- Hot-Start PCR Mix with loading dye, 1300 µl
- Sterile Water 1500 µl
The components are enough for about 100 reactions (25µl/reaction)
Storage: -20°C for about 12 months
Transport: on blue ice
Additionally required (not included):
0,2 ml PCR Tubes, Thermoblock, Waterbath or Thermocycler, optional for swaps sample 1,5 ml Tubes,
Add to a 0,2 ml PCR Tube:
- 85 µl EXT-Buffer
- 5 µl ENZ-Buffer
10 min @ 50°C
5 min @ 95°C
run PCR-protocol or store @ +4°C for up to 1 month.
|Component||Volume in µl|
|Hot-Start Enz-Mix||12,5 µl|
|Forward primer (10 µM)||0,5 - 1,25 µl|
|Reverse primer (10 µM)||
|Your DNA exctract||
up to 25 µl
Post PCR Analysis:
The PCR-Product contails already two dyes, blue and yellow for direct loading on the gel.
In case of:
- lysate is too thick
- or any problem with pipetting: centrifuge and uses supernatant in PCR-
Reaction; or dilute the lysate 1:5 to
1:10 with TE buffer or Tris-HCl, pH 8.0.
- of non-specific or lack of PCR product dilute the lysate 1:5 to 1:10 with
TE buffer or Tris-HCl, pH 8.0.
Fast and convenient Direct Extraction and Amplification Kit. All components for Extraction, Amplification and Gel loading are included. Safe time and your budget!
Final price excl. shipping costs3
Direct Extraction and PCR Kit