Maximo Taq DNA Polymerase 2X-preMix provides robust PCR performance in a wide range of PCR applications and different templates.
Best value in terms of cost per unit. The optimized mixture of all components reduces pipetting mistakes and ensures repeatable results - every day.
Applications for PCR Mastermix:
- Standard / General PCR
- optimized for high specifity
- High-throughput PCR, automated pipetting, or plate based PCR
- Gene mutation
- T/A cloning
Maximo Taq DNA Polymerase 2X-Mastermix is optimized and ready-to-use mixture of all components for a successful PCR. Only your primers and your DNA
Template has to be added.
Maximo Taq DNA Polymerase 2X-preMix contains a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´ exonuclease activity. The
enzyme consists of a single polypeptide with a molecular weight of 94kDa.
Concentration: the mixture is 2X concentrated (Mg2+: 3,6 mM, 1,8 mM final)
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree
List of components:
0.1U/ul Taq DNA Polymerase, 0.4 mM dATP, 0.4 mM dGTP, 0.4 mM dCTP, 0.4 mM dTTP, 4 mM MgSO4, 20 mM KCl, 16 mM
(NH4)2SO4, 20 mM Tris-HCl, pH8.8
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 2 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination
Transportation: on blue ice
Storage: at -20°C for 24 months